From Taiwan, 9 Metarhizium anisopliae strains were introduced to controll mangrove Acrobasis sp. and effects of forest application were compared. The results found that: ( 1) The pathogenicity of the 9 strains was different to controll Acrobasis sp. larvae, but F061 strains was the strongest as the death rate of Acrobasis sp. was 98.75% and the corrected death rate in laboratory 12 d was 97.56%±1.24%, which were significantly higher than other treatments (P< 0.01), and the LT50 was 7.25 d that was the shortest. (2)The best control effect of Acrobasis sp., for the field application, was 1×109 spore·mL-1 and 2×109 spore·mL-1 of Metarhizium anisopliae F061 strains in mangrove, which were 90. 03%± 1. 74% and 90. 55%± 1. 21%, respectively, and were significantly higher than other treatment group (P < 0.01). Finally, the 1×109 spore·mL-1 of F061 strains was suggested from the analysis of control effect and application cost.