首页|高产纤维素酶菌株的鉴定及其活性特征

高产纤维素酶菌株的鉴定及其活性特征

Identification of strains producing cellulase and its enzyme activity determination

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 维普
  • 万方数据
为分析倒木中具有高产纤维素酶菌株的羧甲基纤维素(CMC)酶活性的大小,以天宝岩国家级自然保护区内长苞铁杉倒木作为研究对象,将菌源通过富集培养、滤纸崩解、CMC酶活测定,分离筛选出具有高效降解纤维素的菌株,并将筛选出的高产纤维素酶菌株进行18S rRNA测序分析鉴定.结果表明,经过初筛、复筛,得出TB4为高产纤维素酶的目的菌株,经鉴定该菌株为小刺青霉,该菌株产纤维素酶的酶活性为243.73 U·mL-1.
Based on cumulation cultivation,filter paper disintegrating,carboxymethyl cellulose (CMC) enzyme activity determination,strains with great capacity in cellulose decomposition were isolated to analyze the CMC enzyme activities of fallen logs in the Tsuga longibracteata W.C.Cheng forest of Tianbaoyan National Nature Reserve.18S rRNA sequencing analysis of the selected stains was carried out and identified as Penicillium spinulosum which the CMC enzyme activity was 243.73 U·mL-1.The P.spinulosum strain had the ability in cellulose degradation in the T.longibracteata forest of Tianbaoyan National Nature Reserve.

cellulose-decomposing microorganismsenzyme activityfallen logTsuga longibracteata W.C.Cheng

简立燕、何东进、刘进山、洪伟、游巍斌、肖石红

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福建农林大学林学院,福建福州350002

天宝岩国家级自然保护区,福建永安366032

纤维素降解菌 酶活性 倒木 长苞铁杉

国家自然科学基金教育部博士科学点基金福建省自然科学基金福建省自然科学基金

313706242010351511000508J01162011J01071

2017

10.13324/j.cnki.jfcf.2017.02.013

森林与环境学报
福建农林大学

森林与环境学报

CSTPCDCSCD北大核心
影响因子：0.964
ISSN：2096-0018
年,卷(期)：2017.37(2)
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