Based on high density genetic linkage map of Eucommia ulmoides Oliver and identified quantitative trait locus (QTLs) for important quantitative traits, using the located molecular markers, molecular detection had been conducted to superior individuals that selected from hybrid progenies in E.ulmoides.By the results of correlation analysis between phenotypic data and the corresponding molecular marker effect value, the molecular marker or combinations with higher detection efficiency were selected for marker assisted selection.Combined with the phenotypic selection, the superior individuals with different target traits were chosen.Six markers or marker combinations were screened, including molecular marker DZ200-350 related to tree height, marker combination(em15me23-360 and em12me11-300) related to ground diameter, marker em31me26-160 related to leaf yield, marker em49me3-150 related to leaf gutta-percha content, marker em10me28-170 related to chlorogenic acid content, marker em7me28-240 related to rutin content.Through correlation analysis results of phenotypic traits in 4~6 years old, it can conclude that 6-year-old tree was suit for early phenotypic selection of E.ulmoides.The same superior individuals that approved of phenotypic selection and molecular marker assisted selection in different traits were more than 55%, up to 90%.Phenotypic selection and molecular marker assisted selection were of high consistency in different traits, illustrating indirectly that the molecular marker assisted selection system was also of high efficiency.The established molecular marker assisted selection system was of great significance to accelerate the breeding process of E.ulmoides.
Eucommia ulmoides Oliversuperior individualquantitative trait loci (QTL)marker-assisted selection
国家科技期刊平台
NSTL
维普
万方数据
