杉木愈伤组织的继代增殖与植株再生
Callus subculture and regeneration of Cunninghamiaa lcneoltaa
黄娟 1吴鹏飞 1程浩 1马祥庆1
作者信息
- 1. 福建农林大学林学院, 福建福州350002;国家林业局杉木工程技术研究中心, 福建福州350002
- 折叠
摘要
通过建立杉木愈伤组织增殖和植株再生体系,旨在为杉木体细胞杂交和遗传转化等基因工程研究提供高效受体系统.以杉木子叶、 下胚轴诱导的愈伤组织为材料,研究了不同激素组合及浓度、 培养条件对愈伤组织的继代及植株再生的影响.结果表明:不同激素组合及浓度对愈伤组织的继代有显著影响,2,4-D有利于降低继代过程中的褐化现象,NAA、KT有利于愈伤组织的继代增殖.DCR+6-BA 1.5 mg·L-1+KT 1.0 mg·L-1+NAA 1.0 mg·L-1和DCR+6-BA 2.0 mg·L-1+KT 15.mg·L-1+NAA 1.5 mg·L-1是愈伤组织继代增殖的最佳培养基.暗培养和800 lx光照强度是愈伤组织继代的最佳培养条件.愈伤组织在继代30～45 d期间内需转移至新培养基.愈伤组织在MS+6-BA 0.5 mg·L-1+KT 1.5 mg·L-1再生芽诱导率最高,诱导率为80％.经过一次继代的愈伤组织比未经过继代的单个再生芽数多,平均可达3～4个.再生苗在1/2MS+IBA 1.0 mg·L-1培养基中生根率高达89.5％.
Abstract
In order to provide efficient receptor system for genetic engineering of somatic cell hybridization and genetic transforma-tion, callus proliferation and plant regeneration system of Cunninghamia lanec olata ( Lamb.) HooK was built.Using the callus which induction from the cotyledon and hypocotyl as materials, the effects of the following factors including different types of hormone concentrations and combinations on callus subculture and plant regeneration were investigated.The results indicated that different hormone combinations have a significant influence on callus subculture.2,4-D is helpful to reduce the browning phenomenon in subculture process.NAA and KT are beneficial to the callus subculture.The optimal medium for callus subculture was DCR+6-BA 1.5 mg· L-1+KT 1.0 mg· L-1+NAA 1.0 mg· L-1 and DCR+6-BA 2.0 mg· L-1+KT 1.5 mg· L-1+NAA 1.5mg · L-1 .The optimal culture conditions were dark and 800lx intensity for callus subculture .Callus were most suitable for transfer to new medium after subculture of 30-45 d.The optimal medium for callus induction regeneration bud was MS+6-BA 0.5 mg· L-1 +KT 1.5 mg· L-1 , the induction rates was 80％.The number of buds regenerated from the callus after one subculture was more than that of the callus without subculture, the average number was 3-4.The optimal medium for rooting of regenerated plant was 1/2MS+IBA 1.0 m·g L-1 in a rate of 89.5％.
关键词
杉木/愈伤组织/继代培养/植株再生Key words
Cunninghamia lacn eolata ( Lamb.) Hook./callus/subculture/plant regeneration引用本文复制引用
基金项目
国家重点研发计划项目(2016YFD0600301)
福建农林大学科技发展基金(KF2015036)
出版年
2018
国家科技期刊平台
NSTL
维普
万方数据