油茶芽苗砧嫁接口愈合与内源激素的关系
Relationship between endogenous hormo ne level and the development of the nurse seedling grafted union in Camellia oleifera
冯金玲 1陈辉 1陈世品 1林文俊 1杨志坚1
作者信息
- 1. 福建农林大学林学院,福建福州350002
- 折叠
摘要
以油茶芽苗砧嫁接口为材料,研究内源激素GA3、IAA、ABA和ZR对油茶芽苗砧嫁接口愈合的作用机理.结果表明,GA3、IAA、ABA和ZR都影响油茶芽苗砧嫁接口的愈合.激素间相互作用中,以GA3/ZR、ZR/IAA和ABA/ZR显著影响愈合,其中ZR/IAA主要影响嫁接口形态建成方向,GA3/ZR和ABA/ZR影响嫁接口的形态形成;嫁接体各组织内源激素相关分析得出,4个内源激素间ZR与GA3相关性最大,并达到极显著水平(P).综合分析得出油茶芽苗砧嫁接可通过GA3调控内源激素水平.结果为油茶芽苗砧嫁接科学研究奠定基础,推广应用提供理论指导.
Abstract
To find out healing mechanism, endogenous hormones, including GA3 , IAA, ABA and ZR, were determined by ELISA during the development of the nurse seedling grafted union in Camellia olefi era.The results showed that GA3 , IAA, ABA and ZR all played an important role in the healing of C.oleifera grafted union.GA3/ZR、 ZR/IAA and ABA/ZR had a significant effect on the healing in the grafted union, among which ZR/IAA mainly regulated the morphological formation direction, GA3/ZR and ABA/ZR affected the morphological formation.The correlation analysis showed that the correlation between ZR and GA 3 was the highest betweenwith these four endogenous hormones , which reached the extremely significant level(P).The level of endogenous hormones can be regulated by GA3 .The results will provide the theoretical guidance for the scientific research and the application of the nurse seedling grafting in C.oleifera.
关键词
油茶/芽苗砧嫁接/内源激素/愈合机制Key words
Camellia oleifera Abel./nurse seedling grafting/endogenous hormone/healing mechanism引用本文复制引用
基金项目
高等学校博士学科点专项科研基金(20123515110010)
中央财政林业科技推广示范项目(2017)
福建省教育厅科研基金(JA15191)
出版年
2018