油茶种仁蛋白双向电泳体系的建立
Two-dimensional gel electrophoresis system establishment of Camellia oleifera kernel
何艺凡 1宋倩倩 1林文俊 1陈世品 1陈辉 1郑国华1
作者信息
- 1. 福建农林大学经济林研究所,福建福州350002
- 折叠
摘要
以普通油茶优良无性系'闽43'成熟种仁为材料,从样品制备、IEF聚焦程序参数设定、SDS-PAGE凝胶浓度3个方面优化油茶种仁双向电泳体系.结果表明:采用TCA丙酮+SDS酚抽法提取油茶种仁蛋白能够得到更多较为清晰的蛋白点;采用62000 V·h的高压进行等电聚焦更适合油茶种仁蛋白的第一向分离;10％SDS凝胶浓度更适合油茶种仁蛋白的双向分离,试验结果为深入开展油茶种仁蛋白质组学的研究奠定基础.
Abstract
Using kernel of Camellia oleifera 'Min 43 ' , a proper two-dimensional electrophoresis system for kernel of Camellia oleifera Abel.was optimized by three factors:sample preparation methods, IEF focusing program parameter settings and SDS-PAGE gel concentration.The results showed that more clear spots could be obtained by the method of TCA/acetone combined with SDS phenol extraction, the isoelectric focusing of 62000 V· h was more suitable for the separation of protein and the concentration of 10％SDS gel was better for the separation kernel of C.oleifera protein.The result of this research laid the foundation for the following proteomics research in oil tree.
关键词
油茶种仁/蛋白/双向电泳/体系Key words
Camellia oleifera kernel/protein/two-dimensional electrophoresis/system引用本文复制引用
基金项目
高等学校博士学科点专项科研基金(20123515110010)
中央财政林业科技推广示范项目(2017)
出版年
2018
国家科技期刊平台
NSTL
维普
万方数据