Effects of multi-layer forest management on leaf phenotype and anatomical characteristics of Phoebe bournei
The leaves of Phoebe bournei under different management modes(TN40 and TN60 treatments)of Chinese fir and P.bournei multi-layer forest were used as research materials to investigate the effects of different treatments on the phenotypic traits,anatomical structure,and stomatal morphology of shade and sun leaves of P.bournei.Our findings demonstrated that from the perspective of different leaf types in the same light environment,the leaf thickness,specific leaf weight,palisade tissue,and midrib thickness of the sun-grown leaves of P.bournei under TN40 treatment significantly(P<0.05)increased by 13.77%,9.86%,7.49%,and 11.79%,respectively compared with those of shaded leaves.The leaf thickness,leaf tissue density,specific leaf weight,and stomatal density of the sun leaves of P.bournei under TN60 treatment significantly(P<0.05)increased by 6.32%,9.46%,8.70%,and 6.42%,respectively compared with those of shade leaves.From the perspective of different light environments for the same leaf type,the leaf dry matter content,specific leaf weight,upper epidermis thickness,palisade tissue thickness,sponge tissue thickness,midrib thickness,and stomatal density of the sun-growing leaves of P.bournei under TN40 treatment were 6.13%,4.00%,11.62%,12.17%,15.22%,8.80%,and 9.00%significantly(P<0.05)higher than those under TN60 treatment,respectively;however,the leaf water content and tissue density significantly(P<0.05)decreased by 5.04%and 3.97%,respectively.The leaf area,leaf dry matter content,and stomatal density of the shaded leaves of P.bournei under TN40 treatment were 5.52%,5.50%,and 11.25%significantly(P<0.05)higher than those under TN60 treatment,respectively.Therefore,the morphological adaptation characteristics of P.bournei leaves under TN40 treatment is more suitable for the cultivation of P.bournei.