The study aimed to reveal the basic structure and protein coding information of the chloroplast genome of Chunia bucklandioides,with the aim of providing a reference for future research on its molecular markers,genetic engineering,and genetic diversity.Based on high-throughput sequencing data,the complete chloroplast genome sequence of C.bucklandioides was assembled and analyzed.The total length of the genome was 159 801 bp,with a typical tetrad structure.The GC content of the genome was 38.1％,and the number of effective codons(ENC)was 35.19-54.38.The genome contained 131 coding genes,including 80 protein-coding genes,8 rRNAs,and 37 tRNAs.In addition,71 simple repeated sequence loci were identified.Neutral,ENC,and PR2 plots revealed that the protein-coding genes of the chloroplast genome of C.bucklandioides were mainly influenced by natural selection during codon selection,and tended to use A and U bases for encoding.There were 29 high-frequency codons with relative synonymous codon usage＞1,of which 28 ended at A or U.GCU,GAU,GGA,AAU,CCU,AGA,UCA,and ACA were selected as the optimal codons.Assembly and analysis of the chloroplast genome of C.bucklandioides revealed its tetrad structure,gene composition,and codon usage preference;clarified the dominant role of natural selection in codon selection;and showed a high preference for A and U end codons.
维普
万方数据
