Analyses of chloroplast genome characteristics and codon bias in Chunia bucklandioides
The study aimed to reveal the basic structure and protein coding information of the chloroplast genome of Chunia bucklandioides,with the aim of providing a reference for future research on its molecular markers,genetic engineering,and genetic diversity.Based on high-throughput sequencing data,the complete chloroplast genome sequence of C.bucklandioides was assembled and analyzed.The total length of the genome was 159 801 bp,with a typical tetrad structure.The GC content of the genome was 38.1％,and the number of effective codons(ENC)was 35.19-54.38.The genome contained 131 coding genes,including 80 protein-coding genes,8 rRNAs,and 37 tRNAs.In addition,71 simple repeated sequence loci were identified.Neutral,ENC,and PR2 plots revealed that the protein-coding genes of the chloroplast genome of C.bucklandioides were mainly influenced by natural selection during codon selection,and tended to use A and U bases for encoding.There were 29 high-frequency codons with relative synonymous codon usage＞1,of which 28 ended at A or U.GCU,GAU,GGA,AAU,CCU,AGA,UCA,and ACA were selected as the optimal codons.Assembly and analysis of the chloroplast genome of C.bucklandioides revealed its tetrad structure,gene composition,and codon usage preference;clarified the dominant role of natural selection in codon selection;and showed a high preference for A and U end codons.
万方数据
维普
