Molecular regulation of fatty acid synthesis in Elaeagnus mollis
Elaeagnus mollis is an important woody oil species,the kernel oil of which is characterized by high levels of unsaturated fatty acids,including oleic and linoleic acids.However,the mechanisms underlying the molecular regulation of fatty acid biosynthesis in E.mollis have yet to be sufficiently determined.In this study,gas chromatography-mass spectrometry(GC-MS)and transcriptomic analyses were conducted to quantify the content and molecular regulation of fatty acids in E.mollis kernels at different stages of development.The results indicated that during the late stages of development,unsaturated fatty acids constituted over 90％of the total fatty acids in E.mollis kernels.Specifically,the content of oleic and linoleic acids was as high as 56.03 mg·g-1 and 56.69 mg·g-1,accounting for 42.35％and 42.85％of the total fatty acids,respectively.Transcriptomic analysis revealed 436 genes associated with lipid metabolism pathways,178 genes involved in fatty acid synthesis,and 2 735 genes associated with carbohydrate metabolic pathways.Weighted gene co-expression network analysis(WGCNA)revealed a significant positive correlation of the MEturquoise module with oleic and linoleic acid contents(P＜0.05).The co-expression network analysis indicated that EmWRI1 is an important regulator of fatty acid biosynthesis in E.mollis.The findings of this study enhance the understanding of the biosynthesis genes and key regulators of fatty acids in E.mollis.These findings are of particular scientific and practical value for the optimization of fatty acid composition and the development of high-value-added functional foods,providing a basis for further genetic breeding.
万方数据
维普
