首页|大叶桉根、茎、叶转录组比较和AP 2/ERF基因家族分析

大叶桉根、茎、叶转录组比较和AP 2/ERF基因家族分析

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2021年对广西六峰山林场的大叶桉(Eucalyptus robusta)进行采样,采用Hiseq 2000对大叶桉根、茎、叶组织分别进行转录组测序.结果获得40786个Unigene;通过数据库比对,共注释了 31662个Unigene.GO注释结果最多的条目为膜组成部分;KEGG注释结果表明,5654条序列参与129条代谢通路,参与基因最多的通路为核糖体.对比分析大叶桉根部和茎,以及根部和叶之间的转录差异,发现多条富集的通路,包括α~亚麻酸代谢和萜类骨架生物合成等.鉴定出AP 2/ERF家族基因84个,其中AP 2家族含有9个基因,RAV家族含有2个基因,ERF家族包含73个基因,各基因家族在根茎叶中的表达模式有所差异.
Transcriptome Comparison and AP 2/ERF Gene Family Analysis of Eucalyptus robusta Root,Stem and Leaf
In 2021,Eucalyptus robusta was sampled from Liufengshan forestry center in Guangxi Province.Transcriptome sequencing was performed on the root,stem and leaf tissues of E.robusta by Hiseq2000.40786 unigenes were obtained.A total of 31662 unigenes were annotated by database alignment.The items with the most GO annotation results were membrane components.The KEGG anno-tation results showed that 5654 sequences participated in 129 metabolic pathways,and the pathway with the most genes was ribo-some.A comparative analysis of the transcriptional differences between the roots and stems of E.robusta,as well as between the roots and leaves,revealed multiple enriched pathways,including α-Linolenic acid metabolism and terpene backbone biosynthesis.84 AP 2/ERF family genes were identified,including 9 genes in AP 2 family,2 genes in RAV family and 73 genes in ERF family.Each gene family has different expression patterns in root,stem and leaf.

Eucalyptus robustatranscriptome comparisonAP 2/ERF gene family

陈欢、张健、黄恒成、唐正涛、韦联健、周韬、邓紫宇

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广西林业集团有限公司桂钦林浆纸有限公司,广西南宁 530022

海南大学林学院,海南海口 570228

广西壮族自治区林业科学研究院、广西南宁桉树森林生态系统定位观测研究站、广西优良用材林资源培育重点实验室,广西南宁 530002

大叶桉 转录组比较 AP 2/ERF基因家族

广西壮族自治区林业科技推广示范项目海南省重点研发计划

桂林科研[2022]第10号ZDYF2020046

2024

福建林业科技
福建省林学会,福建省林业科学研究院

福建林业科技

影响因子:0.528
ISSN:1002-7351
年,卷(期):2024.51(1)
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