利用特异性引物进行PCR扩增和克隆、测定不同产区麦冬和山麦冬原植物的18S～26S r DNA-ITS区碱基序列,结果得到供试的7个不同产区麦冬和山麦冬植株样品rDNA的ITS及5.8S rDNA全序列、18S和26S rDNA部分序列,序列长度在696～711 bp.通过分析比较各ITS序列,表明不同产区麦冬和山麦冬rDNA-ITS区存在差异,可为其分子鉴别提供了依据.
rDNA-ITS of Radix ophiopogonis and Radix liriopes from different regions
The 18S-26S rDNA-ITS base sequences of Radix ophiopogonis and Radix liriopes from different geographical regions were compared by using the specific PCR-ITS. The complete sequences of ITS and 5. 8S rDNA, as well as partial sequences of 18S rDNA and 26S rDNA, in the range of 696-711 bp for the seven samples were obtained. By comparing the sequences, it was apparent that the ITS sequences of R. ophiopogonis and R. liriopes from different regions varied. Our analytical results provided a basis for molecular identification of the species.
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