首页|台湾黑僵菌F061菌株孢外酶活性及其对柑橘粉蚧毒力的相关性

台湾黑僵菌F061菌株孢外酶活性及其对柑橘粉蚧毒力的相关性

扫码查看
室内测定台湾黑僵菌F061菌株的孢外酶活性及其对柑橘粉蚧的毒力,并通过线性回归分析探讨两者的相关性.结果表明:供试菌株的2种胞外酶活性均在1.00×108和1.00×107孢子·mL-12个处理时较高,其中蛋白酶活性达7.63U·mL-1和7.61U·mL-,几丁质酶活性达5.45 U·mL-1和5.67U·mL-1;供试菌株对柑橘粉蚧的LT50也在1.00×108和1.00×107孢子·mL-12个处理时较短,分别为4.98d和5.39 d;线性回归分析表明,供试菌株对柑橘粉蚧的L T50与菌株蛋白酶活性呈显著负相关,与菌株几丁质酶活性呈中度负相关.可见,台湾黑僵菌F061菌株蛋白酶活性比几丁质酶活性强,在侵染柑橘粉蚧过程中起更直接的作用.
Correlation Between Ectoenzyme Activity and Virulence against Planococcus citri of Metarhizium anisopliae F061
The ectoenzyme activity and the virulence against Planococcus citri of Metarhizium anisopliae F061 were determined in the laboratory.Their correlation was established using a linear regression analysis.The activities of two ectoenzymes from the bacteria were high.At concentrations of 1.00 × 108 and 1.00)× 107 conidia · mL 1,the protease activities of the spores were 7.63 U · mL-1 and 7.61 U · mL-1,respectively;while the chitinase activities,5.45 U · mL-1 and 5.67 U · mL 1,respectively;and,the LT50 of the strain against P.citri,4.98 d and 5.39 d,respectively.The LT50inversely correlated significantly with the protease activity,but moderately with the chitinase activity.It appeared that the protease were stronger than the chitinase in M.anisopliae F061,and thus,played a more critical role in infectingP.citri.

Metarhizium anisopliaeectoenzymePlanococcus citrivirulencecorrelation

黄鹏、姚锦爱、林勇文、余德亿

展开 >

福建省农业科学院植物保护研究所,福建福州 350013

福建省作物有害生物监测与治理重点实验室,福建福州 350012

福建农林大学植物保护学院,福建 福州 350002

台湾黑僵菌 孢外酶活性 柑橘粉蚧 毒力 相关性

福建省科技计划项目——省属公益类科研院所基本科研专项国家星火计划项目福建省区域发展项目福建省农业科学院科青年人才创新基金

2016R1023-82015GA7200072015N30102014CX-12

2017

福建农业学报
福建省农业科学院

福建农业学报

CSTPCD北大核心
影响因子:0.656
ISSN:1008-0384
年,卷(期):2017.32(1)
  • 2
  • 7