[Objective]Functions of a peptide transporter protein gene,OsPtr1,in rice were studied by means of T-DNA insertion mutation.[Method]The target RNA was extracted from the endosperm of Minghui 86 rice 12 d after flowering and reverse-transcribed into cDNA.By cloning the candidate gene P0421H01.23,named OsPtr1,and constructing the plant overexpression vector using the promoter Gt1 of endosperm-specific expressing,OsPtr1 was introduced into Nipponbare rice using an agrobacterium-mediated method.Nitrogen metabolism of the offspring and mutant w9101 was analyzed.A fusion expression vector of green fluorescent protein gene,GFP,and OsPtr1 was constructed and transferred to onion epidermal cells with a gene gun.In 24-48 h afterward,subcellular localization of OsPtr1 was examined under a fluorescence confocal electron microscope.[Result]OsPtr1 was localized at the membrane and possibly involved in the transport of nitrogen substances.Knockout on OsPtr1 could cause a reduction on nitrogen-containing substances,such as amino acids and proteins,in the rice grains.Conversely,an overexpressed OsPtr1 would increase the accumulation.[Conclusion]OsPtr1 participated in the transmembrane transport of nitrogen substances in rice.
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