首页|福建省山药初级核心种质的构建

福建省山药初级核心种质的构建

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
[目的]构建福建省山药初级核心种质,为福建地方山药资源的保存、品种选育提供理论依据.[方法]以55份福建省地方主栽山药资源为材料,调查 20个农艺性状,包括 7个数量性状和 13个描述型性状,采用离差平方和法进行聚类,根据聚类结果采用优先取样法构建初级核心种质,同时采用统计学方法及ISSR分子标记等对其代表性进行评价.[结果]构建的初级核心种质数量占原种质的 43.6%,在降低冗余的同时保留了选育品种和有突出特点或地方特色的资源.初级核心种质和原种质各性状的均值、方差、变异系数、香农多样性指数无显著性差异.数量性状除茎粗外,核心种质保留了原种质变异范围的 84.9%～100%.描述性指标除最弱的生长势外,核心种质保留了原种质的全部等级分布.初级核心种质和原种质的ISSR分子标记的等位基因数量、有效等位基因数量、香农多样性信息指数、Nei's基因多样性指数无显著差异,且初级核心种质的多态位点保留率达到原种质的 98.6%.基于农艺性状的主成分分析和基于ISSR分子标记的主坐标分析均确认了核心种质的代表性.[结论]构建的福建省山药初级核心种质能够较好地代表原种质的遗传多样性,有助于福建地方山药资源的保存和开发利用.
Establishing a Primary Core Collection of Chinese Yam Germplasms in Fujian
[Objective]A primary core collection of Chinese yam germplasms in Fujian was established for conserving and breeding the natural resources.[Method]Fifty-five locally cultivated Chinese yams were categorized according to 7 quantitative and 13 descriptive traits.A cluster analysis was conducted on the data using the squared Euclidean distance method to develop a germplasm collection by priority sampling.The representativeness of the Primary Core Collection on Chinese Yams(PCCCY)was statistically evaluated using the agronomic traits as well as ISSR molecular markers.[Result]PCCCY received 43.6%of the previous collection.It reduced the redundancy of the old version but retained the breeding varieties as well as the germplasms of outstanding characteristics or unique local features.No significant differences were found in the means,variances,coefficients of variation,and Shannon-Wiener index between the PCCCY and the previous collection on various traits.On the quantitative traits,other than stem thickness,the PCCCY preserved 84.9%to 100%of the variation range of the previous collection.Whereas the entire original descriptive traits,except the weakest growth vigor,were kept intact.No significant differences in the number of ISSR molecular marker alleles,effective alleles,Shannon-Wiener index,and Nei's genetic diversity index were found between the two collections.And 98.6%of the polymorphic loci in the original version were retained.The principal component analysis based on agronomic traits and the principal coordinate analysis based on ISSR molecular markers confirmed a high representativeness on the varieties by the newly established PCCCY.[Conclusion]The primary core collection established by this study for the Chinese yam germplasms in the province satisfactorily represented the genetic diversity of the natural resource.The PCCCY was deemed to facilitate future efforts in conserving and utilizing the local Chinese yam species.

Chinese yamprimary core collectionagronomic traitsISSR markerFujian Province

张武君、陈菁瑛、刘保财、赵云青、黄颖桢

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福建省农业科学院作物研究所/福建省农业科学院药用植物研究中心,福建 福州 350013

山药 初级核心种质 农艺性状 ISSR标记 福建省

福建省农业种质资源创新专项福建省药用植物种质资源库(圃)项目福建省农业高质量发展超越"5511"协同创新工程项目福建省农科院药用植物科技创新团队建设项目福建省农科院生产性工程化实验室建设项目

ZZZYCXZX202208ZYBHDWZX202203XTCXGC2021003-5CXTD2021014-22015GCH-6

2023

10.19303/j.issn.1008-0384.2023.11.002

福建农业学报
福建省农业科学院

福建农业学报

CSTPCDCSCD北大核心
影响因子：0.656
ISSN：1008-0384
年,卷(期)：2023.38(11)
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