首页|粉皮冬瓜表皮蜡粉微形态及蜡粉组分研究

粉皮冬瓜表皮蜡粉微形态及蜡粉组分研究

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
[目的]探明不同粉皮冬瓜品种表皮蜡粉成分特性,为进一步解析冬瓜表皮蜡粉的分子调控奠定基础.[方法]选择形状不同的 2个粉皮冬瓜品种(夏茂粉皮小冬瓜和夏茂粉皮大冬瓜)为试验材料,采用扫描电子显微镜观察表皮蜡粉结构,通过非靶向气相色谱-质谱(GC-MS)检测技术对表皮蜡粉的成分进行分析.[结果]夏茂粉皮小冬瓜和夏茂粉皮大冬瓜表皮均有大量堆叠的杆状蜡粉结构,但夏茂粉皮小冬瓜杆状的前端上还粘附丝状蜡粉.2个品种三萜类物质含量均占比最高(42.09%和 35.63%),但酯类和烷烃物质含量占比存在明显差异,夏茂粉皮小冬瓜分别为 31.16%和 3.53%,夏茂粉皮大冬瓜分别为 22.17%和 17.34%.在检测出的 9类 65种成分中,11种化合物在 2个品种间呈极显著差异,8种化合物呈显著差异.[结论]冬瓜表皮蜡粉形态结构和成分在不同品种间存在较大差异,不同的蜡粉成分对其结构的形成有重要影响.通过对不同冬瓜品种表皮蜡粉成分的鉴定,为今后明确蜡粉性状在冬瓜中的遗传规律,阐明其遗传分子机制,具有重要的科研和生产实践价值.
Micro-morphology and Compositions of Epidermal Wax on Waxy Gourd
[Objective]Micro-morphology and chemical composition of the wax in pericarps of two varieties of waxy gourd were examined.[Method]The wax on the pericarps of the differently shaped Xiamaofenpixiaodonggua(XFX)and Xiamaofenpidadonggua(XFD)waxy gourds were examined under a scanning electronic microscope.The epidermal wax composition was analyzed by non-targeted gas chromatography-mass spectrometry(GC-MS)metabolomics.[Result]Numerous stacked rod-like structures covered the surface of XFX and XFD,but the filamentous material appeared only on XFX.Triterpenoids were the most abundant compounds found in the wax,i.e.,42.09%on XFX and 35.63%on XFD.On the other hand,the esters and alkanes differed significantly between the two varieties,which were 31.16%and 3.53%,respectively,on XFX,and 22.17%and 17.34%,respectively,on XFD.In total,65 substances in 9 categories were identified that included 11 extremely significantly and 8 significantly differentiated compounds.[Conclusion]The micro-structures and compositions of the epidermal wax of the two waxy gourd varieties were surprisingly different,and the structural differentiation could be chemically related.Further study to decipher the biosynthesis pathway of the waxy gourd is in order.

Pericarp waxwaxy gourdsGC-MSstructurecomponent

张荟、叶新如、朱海生、温庆放

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福建省农业科学院作物研究所,福建 福州 350013

福建省蔬菜遗传育种重点实验室,福建 福州 350013

福建省蔬菜工程技术研究中心,福建 福州 350013

表皮蜡粉 冬瓜 气相色谱-质谱法 结构 成分

福建省农科院自由探索科技创新项目福建省农科院科技创新团队建设项目国家大宗蔬菜产业技术体系建设项目福州综合试验站项目福建省种业创新与产业化工程项目福建省农业高质量发展超越"5511"协同创新工程项目

ZYTS202206CXTD2021003-1CARS-23-G51zycxny2021009XTCXGC2021003

2023

10.19303/j.issn.1008-0384.2023.11.003

福建农业学报
福建省农业科学院

福建农业学报

CSTPCDCSCD北大核心
影响因子：0.656
ISSN：1008-0384
年,卷(期)：2023.38(11)
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