[Objective]A series of new rice germplasms with high content of amylose derived from Zhonghua 11 using the CRISPR/Cas9 technology was generated.[Method]The gene of starch branching enzyme in rice,OsSBE3,was targeted for the genetic editing by CRISPR/Cas9.Homozygous T-DNA-free mutants were verified by PCR with starch content measured.[Result]Ten mutant lines were obtained from the T0 generation.From the T1 generation,5 homozygous T-DNA-free lines were obtained that included 4 lines,i.e.,sbe3-22-6,sbe3-25-3,Sbe3-25-4,and sbe3-25-6,showing significantly increased amylose content and amylose/amylopectin ratio over the wild type.[Conclusion]A series of new rice germplasms with high amylose content was created.
维普
万方数据