Characteristics of SSRs in Zicaitai Mitochondrial Genome
[Objective]The simple sequence repeats(SSRs)in the mitochondrial genome of zicaitai(Brassica rapa var.Purpuraria)were compared with those of other species in the genus.[Method]MISA software was used to search the mitochondrial genomes of zicaitai as well as 5 other major species and 3 mutants of Brassica rapa.The SSR sequence distributions of the specimens were compared and analyzed.[Result]In the mitochondrial genomes,168,170,167,168,180,280,185,165,and 179 complete SSRs were screened.The sequences showed relative densities of 764,774,760,764,775,777,721,744,and 771 per Mb with total lengths of 1562,1577,1547,1562,1664,2564,1722,1524,and 1646 bp that accounted for 0.71%,0.72%,0.70%,0.71%,0.72%,0.71%,0.67%,0.69%,and 0.71%of the total sequence length of each specimen,respectively.Among the 1-6 different nucleotide repeat units in zicaitai and the other major Brassica species,most SSRs were mononucleotide,which was followed by dinucleotide,tetranucleotide,trinucleotide,and pentanucleotide,but no hexonucleotide.A/T,AG/CT,AT/AT,and C/G were the common repeat unit types in them.[Conclusion]The mitochondrial genome of zicaitai was 219779 bp with 168 SSRs at the relative density of 764 markers/Mb and 9 bp in length.Mono-,di-,and tetra-nucleotides were the most abundant repeat units,which could potentially be developed as highly polymorphic markers for species differentiation.
万方数据
维普
