[Objective]To utilize a Pseudostellaria heterophylla by-product for increasing Antrodia camphorata yield and bio-effectiveness,formulation of a culture medium was optimized.[Methods]Powder of P.heterophylla fine roots was included in formulating a medium to maximize the mycelial growth of A.camphorata by a Plackett-Burman orthogonal experimental design.[Results]In addition to 6 g·L-1 of P.heterophylla powder,the medium was optimized to contain the ingredients critical to the mushroom growth,which included glucose 10 g·L-1,yeast extract 4 g·L-1,peptone 6 g·L-1,and KH2PO41.5 g·L-1.The A.camphorata culture on the formulated medium produced 4.73 g·L-1 biomass,which yielded 2.75%more intracellular triterpenoids than control and 24.85%more than PDB medium,and 161.11%more exopolysaccharides than control and 113.64%more than PDB medium.The fermentation broth also contained a high asexual spore at a density of 1.8×107 spores·mL-1.[Conclusion]P.heterophylla root powder could plausibly be added to the medium for A.camphorate culture as it promoted the mycelial growth,bioactive constituents yield,and asexual spore production of the mushroom.
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