首页|一株具有插入和缺失突变特征鸭腺病毒B2毒株的分离鉴定

一株具有插入和缺失突变特征鸭腺病毒B2毒株的分离鉴定

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
[目的]对一份疑似鸭腺病毒B2(Duck adenovirus B2，DAdV B2)感染的番鸭白肝病的病例进行确诊，并对分离株进行测序，为福建省DAdV B2流行病学研究提供参考。[方法]开展病料PCR检测，并进行病毒的分离鉴定、全基因二代测序，利用MegAlign和SnapGene软件对测序结果进行同源性及遗传进化分析，再进行动物回归试验，确定对雏番鸭的致病性。[结果]病料样本检测为DAdV B2阳性，并成功分离到一株DAdV B2毒株，命名为DAdV B2/BG48。该分离株感染鸡肝癌细胞(LMH)后细胞变大变圆、最后死亡崩解，形成特征性细胞病变;感染MDEF后细胞由长梭形变为圆形并聚集，细胞间出现空隙。测序结果表明，BG48基因组在pX基因区域中有 3bp的插入，在ORF19B基因区域有 33 bp的插入，在ORF64和ORF67基因区域的交界处有 42 bp的缺失，ORF67基因起始密码子往后第 133位碱基为G，没有突变为终止密码子，其他编码基因与CH-GD-12-2014、实验室之前鉴定的BG27和BG18无特征性差异。动物回归试验显示，2日龄的番鸭对DAdV B2分离株BG48易感，致病率为 50%，死亡率为 0，发病鸭可见与自然感染病鸭相似的临床症状和病理变化。[结论]成功从番鸭白肝病病例中分离鉴定 1株DAdV B2突变株BG48，BG48具有多位点插入和缺失特征，提示DAdV B2毒株容易突变，临床流行毒株复杂。本结果为DAdV B2的分子流行病学调查和遗传进化研究提供了参考。
Isolation and Identification of a New Duck Adenovirus B2 with Insertion and Deletion Mutations
[Objective]A previously unknown strain of duck adenovirus B2(DAdV B2)was isolated from a ducking of pale liver disease in Fujian and identified for epidemiological reference.[Methods]The virus associated with the disease was detected by PCR and isolated for identification by genome sequencing and a challenge test.[Result]The DAdV B2/BG48 virus infected LMH cells in the duck became swollen like a ballon,and eventually,died and disintegrated.The MDEF cells inoculated with the isolate changed from a spindle-shape to ball-like and clustered together with intercellular spaces in between.Distinctively,BG48 genome had a 3 bp insertion in the pX region,a 33 bp insertion in the ORF19B domain,and 42 bp deletion at the junction between ORF64 and ORF67.A G-base was found on the 133rd spot after the starting code of ORF67 and no truncated mutation in the terminal nor other codes compared with CH-GD-12-2014,or BG27 and BG18 previously identified.In a challenge test,BG48 induced a 50%morbidity and 0 mortality on 2-day-old Muscovy ducks with similar clinical symptoms and pathological changes between the artificially infected and the naturally diseased birds.[Conclusion]A novel DAdV B2 strain,BG48,from a Muscovy duck of pale liver disease was isolated and identified.The virus was uniquely characterized by multiple insertions and deletion mutations in the gene suggesting a potential of causing complex epidemic.

Pale liver diseaseduck adenovirus B2isolation and identificationInsertion and deletionORF67

郭博伦、李雅汝、郑欣、江丹丹、曾丽、陈少莺、马燕梅、陈仕龙

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福建农林大学动物科学学院/福建省-尼泊尔畜禽重大疫病防控联合实验室,福建 福州 350002

福建省农业科学院畜牧兽医研究所,福建 福州 350013

福建省畜禽疫病防治工程技术研究中心,福建 福州 350013

番鸭白肝病 鸭腺病毒B2 分离鉴定 插入和缺失 ORF67

福建省科技计划公益类专项福建省农科院科技创新团队建设项目福建省农业高质量发展超越"5511"协同创新工程项目福建省农业高质量发展超越"5511"协同创新工程项目福建农林大学科技创新专项

2019R1026-11CXTD2021034XTCXGC2021018XTCXGC2021012KFB23098A

2024

10.19303/j.issn.1008-0384.2024.02.001

福建农业学报
福建省农业科学院

福建农业学报

CSTPCD北大核心
影响因子：0.656
ISSN：1008-0384
年,卷(期)：2024.39(2)
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