摘要
[目的]明确河南信阳地区白术根腐病病原菌种属,研究其生物学特性并筛选可用于防控其病原菌的植物源农药.[方法]从种植区采集病株,室内分离病原菌并纯化,利用形态学和多基因联合分析对病原菌进行鉴定,并对病原菌的生物学特性进行研究,利用菌丝生长速率法评价 3种植物源杀菌剂对其菌落的抑制作用.[结果]该病原菌菌丝白色,孢子呈卵圆形,两端稍尖,大型分生孢子 3～5隔,大小为(7～10)μm×(3～4)μm,小型分生孢子 2隔或无格,大小为(3～5)μm×(1～2)μm,多基因联合分析表明该病原菌与藤仓镰刀菌(Fusarium fujikuroi)同源性高,结合形态学特征与分子生物学分析,将信阳白术根腐病病原菌鉴定为藤仓镰刀菌(Fusarium fujikuroi),这是藤仓镰刀菌引起白术根腐病的首次报道.生物学特性研究显示,该病原菌最适培养条件为温度 28℃、pH为 7,最适生长碳源为蔗糖,最适生长氮源为硝酸钾.3种植物源杀菌剂中,0.3%丁子香酚可溶液剂的EC50 为 6.906 mg·L-1,高于其他供试药剂,对病原菌的毒力最强.[结论]河南信阳地区白术根腐病病原菌为藤仓镰刀菌,该研究结果为信阳地区白术根腐病科学防控提供了依据.
Abstract
[Objective]Pathogen that caused the root rot on Atractylodes macrocephala at Xinyang,Henan Province was isolated,identified,and studied for prevention and control of the disease.[Method]Morphological and multiple gene analyses were employed to identify the pathogen.Biological characteristics of the isolate were determined.[Result]The isolate had white hyphae,oval spores with slightly pointed ends,macroconidia sized(7-10)μm×(3-4)μm with 3-5 septa,and microconidia sized(3-5)μm×(1-2)μm with two or no compartments.It showed a high homology with Fusarium fujikuroi and was,for the first time,identified as the fungal pathogen responsible for the root rot disease on Atractylodes macrocephala.It thrived at 28℃,pH 7,sucrose for carbon,and potassium nitrate for nitrogen.Of the 3 plant-based fungicides tested,0.3%eugenol soluble agent exerted the greatest toxicity on the isolate with an EC50 of 6.906 mg·L-1.[Conclusion]F.fuciformis was identified as the pathogen that caused the root rot disease on A.macrocephala in Henan.The optimal culture conditions for the fungus included 28℃,pH 7,sucrose as the carbon source,and potassium nitrate as the nitrogen source.
基金项目
河南省高等学校重点科研项目(21B210009)
信阳农林学院青年教师科研基金(QN2021060)
河南省科技攻关计划(222102110247)
国家科技期刊平台
NSTL
万方数据