Isolation,Identification,Biological Characteristics,and Fungicide Toxicity of Atractylodes Root Rot Pathogen
[Objective]Pathogen that caused the root rot on Atractylodes macrocephala at Xinyang,Henan Province was isolated,identified,and studied for prevention and control of the disease.[Method]Morphological and multiple gene analyses were employed to identify the pathogen.Biological characteristics of the isolate were determined.[Result]The isolate had white hyphae,oval spores with slightly pointed ends,macroconidia sized(7-10)μm×(3-4)μm with 3-5 septa,and microconidia sized(3-5)μm×(1-2)μm with two or no compartments.It showed a high homology with Fusarium fujikuroi and was,for the first time,identified as the fungal pathogen responsible for the root rot disease on Atractylodes macrocephala.It thrived at 28℃,pH 7,sucrose for carbon,and potassium nitrate for nitrogen.Of the 3 plant-based fungicides tested,0.3%eugenol soluble agent exerted the greatest toxicity on the isolate with an EC50 of 6.906 mg·L-1.[Conclusion]F.fuciformis was identified as the pathogen that caused the root rot disease on A.macrocephala in Henan.The optimal culture conditions for the fungus included 28℃,pH 7,sucrose as the carbon source,and potassium nitrate as the nitrogen source.
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