SERS Detection of Enrofloxacin and Ciprofloxacin Residues in Seafood
[Objective]A rapid method for detecting residues of enrofloxacin(ENR)and ciprofloxacin(CIP)in seafood by surface-enhanced Raman spectroscopy(SERS)was developed.[Method]On spiked seafood samples,(50±5)nm silver nanoparticles were used as the enhancement reagent and 200 g·L-1 NaCl solution as the extraction solution for the SERS assay.Detection results were read within 3 min and mathematical correlation between peak measurement and drug concentration examined.Based on the linearity and limit of detection,optimal assay conditions were determined.[Result]The assay showed several peaks on ENR and CIP standards.The C-N bending vibration caused the peak at 532 cm-1,the out-of-plane bending vibration at 552 cm-1,the C-N,C-C-F out-of-plane and C-C=O in-plane bending vibrations at 651 cm-1,the stretching vibration of C-H on the benzene ring,C-C=O out-of-plane bending vibration,C-C-N bending vibration and C-H,C-N stretching vibration at 737 cm-1,and the C-H,C-N out-of-plane bending vibration at 785 cm-1.By quantifying the characteristic peak at 737 cm-1,a linear relationship with an R2＞0.96 was found between the Raman intensity and drug concentration in the range of 10-200 ng·mL-1.The assay had a recovery rate of 78.4%～106.7%,RSD of 2.1%～6.7%,and a detection limit of 10μg·kg-1.[Conclusion]The newly developed rapid SERS assay for detecting ENR and CIP residues in seafood was deemed accurate,stable,and applicable for field screening and testing.
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