摘要
[目的]甜樱桃(Cerasus avium L.)的S基因决定其自交的亲和性.为鉴定四川地区甜樱桃品种的S基因型和高自交亲和性品种,本研究对收集到的39份甜樱桃材料进行S基因型鉴定,并比较SFB4与其自交亲和突变体SFB4基因的差异.[方法]利用S基因通用引物和特异引物对四川地区种植的39份甜樱桃叶片DNA进行PCR扩增,并对含有SFB4/SFB4'基因的扩增片段进行测序.[结果]39份甜樱桃材料共鉴定出S1、S2、S3、S4、S6、S9等6个基因型,其中27份材料含有S3基因,19份材料含有S9基因,12份材料含有S1基因,11份材料含有S4基因,7份材料含有S6基因,3份材料含有S2基因.根据鉴定得到的S基因型结果,扩增得到含有S4基因的11份材料中的SFB基因的ORF序列,比对结果显示,相比于SFB4,SFB4'在 903 bp处存在4个碱基缺失;本研究设计了 SFB4特异引物,测序结果显示,有7份材料的SFB基因型为SFB4'.[结论]共鉴定得到SI、S2、S3、S4、S6、S9等6个基因型,对含有S4基因的11份材料的S4基因全长进行测序,确定7份材料为突变基因SFB4'.本研究为四川地区甜樱桃授粉树配置提供了数据支撑,并为甜樱桃新品种的选育奠定了理论基础.
Abstract
[Objective]Varieties of sweet cherry cultivars from Sichuan Province were analyzed for self-compatibility by the presence of mutant S gene.[Method]Using the universal and specific primers of S genotype,DNA from the leaves of 39 Cerasus avium L.specimens collected in Sichuan were amplified by PCR.The amplified fragments containing SFB4 and/or SFB4'were sequenced for comparison and further analysis.[Result]Six genes of S genotype were identified from the 39 sweet cherry specimens with 27 of them having S3 genes,19 having S9,12 having S1,11 having S4,7 having S6,and 3 having S2.In the 11 specimens containing S4 genes,the ORF sequences of SFBs were amplified.By comparing SFB4 and SFB4',4 base deletions at 903 bp were observed on the mutant.Subsequently,with a specially designed primer for SFB4',the sequencing indicated the SFB in 7 of the specimens to be SFB4'.[Conclusion]Six S genes were identified in the 39 C.avium specimens collected in Sichuan.The S4 from 7 of the specimens was the mutant gene,SFB4'.
万方数据