Identification of S Genes and SFB4'in Cerasus avium L.
[Objective]Varieties of sweet cherry cultivars from Sichuan Province were analyzed for self-compatibility by the presence of mutant S gene.[Method]Using the universal and specific primers of S genotype,DNA from the leaves of 39 Cerasus avium L.specimens collected in Sichuan were amplified by PCR.The amplified fragments containing SFB4 and/or SFB4'were sequenced for comparison and further analysis.[Result]Six genes of S genotype were identified from the 39 sweet cherry specimens with 27 of them having S3 genes,19 having S9,12 having S1,11 having S4,7 having S6,and 3 having S2.In the 11 specimens containing S4 genes,the ORF sequences of SFBs were amplified.By comparing SFB4 and SFB4',4 base deletions at 903 bp were observed on the mutant.Subsequently,with a specially designed primer for SFB4',the sequencing indicated the SFB in 7 of the specimens to be SFB4'.[Conclusion]Six S genes were identified in the 39 C.avium specimens collected in Sichuan.The S4 from 7 of the specimens was the mutant gene,SFB4'.
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