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瘤胫果实蝇线粒体全基因组分析与系统发育

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[目的]明确瘤胫果实蝇[Bactrocera Bactrocera tuberculata(Bezzi)]线粒体全基因组序列特征，探究其系统发育地位，为瘤胫果实蝇分子标记、物种鉴定和进化遗传学研究提供参考依据。[方法]采用高通量测序技术对瘤胫果实蝇的线粒体全基因组进行测序、组装、拼接和注释，获得基因组全序列，并对基因组基本结构进行分析;选择NCBI公布的果实蝇属近缘种20种实蝇的线粒体全基因组序列，基于最大似然法(Maximum likelihood，ML)进行系统发育分析。[结果]瘤胫果实蝇线粒体基因组序列总长度为15 854bp;具37个基因，其中包含13个蛋白编码基因、22个tRNA基因、2个rRNA基因和1个非编码控制基因。瘤胫果实蝇线粒体基因组全序列A+T含量为73。2％;13个蛋白质编码基因共有密码子3 755个，在蛋白质所有22种氨基酸密码子中，UUA(leucine)的使用频率N及相对密码子RSCU使用频率均最高，N(RSCU)为387(3。79);tRNA基因二级结构中，除苯丙氨酸(F)以及苏氨酸(T)缺少假尿嘧啶(T)环和丝氨酸(S1)缺少二氢尿嘧啶DHU环，其余19个tRNA基因均能折叠形成典型的三叶草二级结构，22个tRNA基因中，共存在178处碱基对错配，错配碱基对均为G-U。通过对其近缘种线粒体全基因组的系统发育分析，瘤胫果实蝇与桔小实蝇(Bactrocera dorsalis)和杨桃实蝇(Bactrocera carambolae)聚在一起，表明3种实蝇的亲缘关系较近，与果实蝇亚属的其他种类聚在同个分支上，结果与形态鉴定一致。[结论]首次报道了瘤胫果实蝇线粒体全基因组，GenBank登录号MW 892726，测定和分析的瘤胫果实蝇线粒体基本结构特征，核苷酸组成、系统进化分析，支持瘤胫果实蝇属于果实蝇亚属，为物种诊断、进化生物学和防治研究提供依据。
Mitochondrial Genome and Phylogeny of Bactrocera(B.)tuberculata
[Objective]Mitochondrial genome and phylogeny of Bactrocera(B.)tuberculata were studied for advancement on the molecular markers design,species identification,and evolutionary genetics relating to the destructive fruit fly.[Methods]High-throughput sequencing was applied to determine,assemble,join,and annotate the complete genome of B.(B.)tuberculata mitochondria.With the sequences on selected 20 species published on NCBI,the phylogeny of fruit flies was analyzed using the maximum likelihood method(ML).[Results]The total mitochondrial genome sequence was 15 854 bp long containing 13 protein coding genes,22 tRNA genes,2 rRNA genes,and one non-coding control gene with 73.2％A+T.There were 3 755 codons in the 13 protein-coding genes.Of the 22 amino acid codons in the protein,UUA(leucine)had the highest frequency(N)of 387 and relative codon(RSCU)of 3.79.Aside from the phenylalanine(F)and threonine(T)that lacked pseuduracine(T)rings and the serine(S1)without a dihydrouracil DHU ring,the secondary structures of the remaining 19 tRNA genes shaped typically like a canonical cloverleaf.And the 22 genes had 178 mismatched G-U base pairs.Based on the mitochondrial analysis,the phylogeny of B.(B.)tuberculate,B.(B.)dorsalis,and B.(B.)carambolae were closely related and in the same branch as other subgenera.The result agreed with what was revealed by the morphological observation.[Conclusion]For the first time,the complete mitochondrial genome of B.(B.)tuberculata was obtained with a GenBank accession number of MW 892726.The information secured on the structure and nucleotide composition of the mitochondria and the phylogenetic relation with other subgenera would aid further studies on the species identification,evolutionary biology,and pest control of the devastating pest.

Bactrocera Bactrocera tuberculata(Bezzi)mitochondrial genomesequence analysisrelative codon usage frequencysecondary structure of tRNA genesphylogeny

黄振、郭琼霞

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福州长乐机场海关,福建 福州 350209

福州海关技术中心,福建 福州 350001

瘤胫果实蝇 线粒体全基因组 序列分析 相对密码子使用频率 tRNA基因二级结构 系统发育

福建省自然科学基金项目福建省自然科学基金项目福州海关科研项目

2011J010662012JO1061fk2022-09

2024

10.19303/j.issn.1008-0384.2024.04.013

福建农业学报
福建省农业科学院

福建农业学报

CSTPCD北大核心
影响因子：0.656
ISSN：1008-0384
年,卷(期)：2024.39(4)