[Objective]Somatic incompatibility (SI) and genetic differences among Ganoderma sinense strains were studied.[Method]Dikaryon G.sinense strains were generated from 7 monokaryon parents by uni-or bi-directional nuclear migration.On a PDA medium,SI between the dikaryon strains was tested,and genetic differentiations examined using ISSR,RAPD,and SRAP molecular markers.[Result]Eleven dikaron strains of 5 distinctive genetic types showing a gap,a gap with line,or a wall-like SI were obtained.The combined genetic similarity coefficients of the ISSR,RAPD,and SRAP molecular markers on the strains ranged from 0.29 to 0.97.The UPMGA clustering of the strains corresponded to that of the parents.[Conclusion]The SI among the G.sinense strains were mainly associated with the nucleus rather than the cytoplasm.Since the SI types largely paralleled the genetic differences of the dikaron strains,SI determination could be conveniently applied to preliminarily study the genetics of a G.sinense strain.
维普
万方数据