Identification and Expression under Salt Stress of NHXs in Brassica napus L.
[Objective]Members of the Na+/H+antiporter(NHX)family in Brassica napus L.were identified,and genes related to salt stress screened.[Methods]The genome of B.napus cv.Zhongshuang 11 was used to obtain the candidate genes,and NHXs sequences by homologous alignment.Physicochemical properties,evolutionary relationship,and expressions under salt stress of the genes were analyzed.[Results]Twenty-one BnNHXs were identified with 71 to 1265 amino acids,an isoelectric(pI)ranging from 5.54 to 7.68,and 0 to 24 introns.Among the BnNHXs,BnNHX1 was in the plasma membrane,BnNHX5 and BnNHX12 in the cell and vacuole membranes,and the remainders in the vacuole only.Phylogenetically,NHXs could be divided into 3 subfamilies scattered on 11 chromosomes containing a variety of abiotic stress response elements.The qRT-PCR expressions on most BnNHXs were upregulated under salt stress,with overall higher expression in the leaves than in the roots.Notably,BnNHX2,BnNHX6,BnNHX8,BnNHX11,and BnNHX19 had relatively more pronounced changes in the expression.[Conclusion]Five NHXs,namely,BnNHX2,BnNHX6,BnNHX8,BnNHX11,and BnNHX19,could be the candidate genes for breeding salt-tolerant new varieties as well as for studying molecular mechanisms of rapeseed.
万方数据
维普
