Physiobiochemical Changes and Gene Expressions of Polygonatum cyrtonema Hua Seeds undergone Post-ripening Treatment
[Objective]Physiobiochemical changes and differentially expressed genes in Polygonatum cyrtonema Hua seeds after a post-ripening treatment were studied.[Method]Contents of superoxide dismutase,α-amylase,β-amylase,soluble protein,crude fat,and soluble sugar at 6 seed development stages were monitored.Differentially expressed genes were analyzed with high-throughput sequencing followed by a qRT-PCR verification.[Result](1)The post-ripening treatment raised the soluble sugar content in the seeds gradually in the initial 4 stages and slightly declined in the last two stages,while induced a declining trend on the crude fat and protein.Meanwhile,the activities of α-amylase and β-amylase increased.(2)Significantly enriched differentially expressed genes were observed in the sucrose and starch metabolic pathways as well as the hormone signal transduction pathway during the process.[Conclusion]The post-ripening treatment on the P.cyrtonema seeds brought about the conversion and metabolism of starch and other nutrients as well as endogenous hormones interactions promoting the physiological changes and dormancy release.
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