Cloning and Preliminary Functional Verification of VfNHX1 in Vicia faba L.
[Objective]Role of VfNHX1 of faba bean in response to salt stress was studied.[Methods]A Na+/H+reverse transporter protein-encoding gene,VfNHX1,was cloned from Vicia faba L.by 3'and 5'RACE for a bioinformatics analysis,subcellular localization determination,expression under salt stress,and a preliminary verification on the function.[Results](1)VfNHX1 sequence was 2255 bp with a CDS coding region of 1629 bp encoding 542 amino acids.(2)As a stable transmembrane protein,VfNHX1 had 10 transmembrane regions without signal peptide and contained a typical Na-H exchanger conserved functional domain of NHX family.It was in the vacuolar membrane.(3)In the leaves,the expression of VfNHX1 under NaCl stress determined by qRT-PCR showed a trend of decreasing,then increasing,followed by decreasing with a peak reached in 12 h.In the roots,the expression declined initially and then rose significantly at 48 h(P＜0.01).(4)The presence of VfNHX1 in a yeast growth experiment indeed heightened the NaCl tolerance of the salt-sensitive yeast mutant AXT4K.[Conclusion]VfNHX1 in V.faba L.was able to respond to salt stress and could be a functional gene to boost the salt resistance of the plant.
万方数据
维普
