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不同紫云英品种根瘤内生细菌的类群和多样性分析

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[目的]阐释紫云英根瘤内生菌的群落结构,为研发根瘤菌菌剂及了解固氮机制提供依据。[方法]以福建省种植的不同紫云英品种的根瘤为材料,借助Illumina Miseq PE300测序平台,以 16S rRNA基因为靶标,研究不同紫云英品种的根瘤内生细菌的多样性。[结果]不同品种会影响紫云英根瘤内生细菌的类群和多样性。浙紫 5号(ZZ5)的优势属为假单胞菌属(Pseudomonas),其余品种根瘤内生细菌的优势属均为中慢生根瘤菌属(Mesorhizobium)。闽紫 8号(MZ8)、信阳(XY)和浙紫 5号的根瘤内生细菌的多样性指数(Shannon)较高,除了共生固氮的中慢生根瘤菌外,还包括丰富的非根瘤菌,如假单胞菌属、寡养单胞菌属(Stenotrophomonas)、杜擀氏菌属(Duganella)、泛菌属(Pantoea)、欧文氏菌属(Erwinia)等。闽紫 8号、信阳和浙紫 5号这 3个品种根瘤中含有较高丰度的致病菌大黄欧文氏菌(Erwinia rhapontici)和菠萝泛菌 PA13(Pantoea ananatis PA13)。[结论]紫云英品种对根瘤内生细菌的群落结构有一定影响,浙紫 5号的根瘤内生细菌主要为具促生作用的非根瘤菌假单胞菌,而其余品种为具固氮能力的根瘤菌,在生产实际中可以采用紫云英混种的方式达到固氮促生的双重功效。
Endophytic Microbes in Root Nodules of Different Cultivars of Astragalus Sinicus L.
[Objective]Being crucial to the development of rhizobium inoculants and the understanding on the associated mechanisms of soil nitrogen fixation,community structure of the endophytic microorganisms in root nodules of Astragalus sinicus L.was investigated.[Method]Endophytic microbial community in the root nodules of 7 cultivars of A.sinicus planted in Fujian was analyzed with the Illumina Miseq PE300 sequencing platform using 16S rRNA as the target gene.[Result]The microbial composition of endophytic community in the nodules of the different cultivars varied.For instance,the predominant genus in ZZ5 root nodules was Pseudomonas,but it was Mesorhizobium in other cultivars.Furthermore,high Shannon indices were found in the root nodules of MZ8,XY,and ZZ5 with diverse non-rhizobial endophytes like Pseudomonas,Stenotrophomonas,Duganella,Erwinia,etc.In contrast,high relative abundances of pathogens,such as Erwinia rhapontici and Pantoea ananatis PA13 existed in the root nodules of MZ8,XY,and ZZ5.[Conclusions]Significant microbial variations in the endophytic communities were evident among the different varieties of A.sinicus planted in Fujian.The predominant genus in the root nodules of ZZ5 was non-rhizobial,growth-promoting Pesudomonas,while it was rhizobia for the other cultivars.

Astragalus sinicuscultivarroot noduleendophytic bacteriadiversity

方宇、刘彩玲、林陈强、陈济琛、贾宪波

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福建省农业科学院资源环境与土壤肥料研究所,福建 福州 350003

紫云英 品种 根瘤 内生细菌 多样性

福建省科技计划公益类专项福建省自然科学基金项目福建省自然科学基金项目福建省农业科学院科技创新团队建设项目福建省农业科学院农业高质量发展超越"5511"协同创新工程项目福建省农业科学院科技创新平台专项现代农业产业技术体系建设专项福建省对外合作项目

2022R10250062023J013602021J01480CXTD2021002-3XTCXGC2021009CXPT2023007CARS-22DWHZ2023-5

2024

福建农业学报
福建省农业科学院

福建农业学报

CSTPCD北大核心
影响因子:0.656
ISSN:1008-0384
年,卷(期):2024.39(6)