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内蒙古赤峰地区辣椒病毒种类鉴定及其遗传进化特征

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[目的]系统检测内蒙古赤峰市辣椒主产区病毒发生种类，并分析病毒的遗传分化情况，以期为该地区辣椒病毒病精准防控提供理论依据。[方法]从内蒙古赤峰地区 4个辣椒主产区采集疑似病毒侵染的辣椒样本47份，采用sRNA高通量测序技术结合生物信息学分析，以及常规RT-PCR检测，明确侵染辣椒的病毒种类及复合侵染情况;采用常规Sanger测序测定常规RT-PCR扩增片段序列，进行遗传进化分析。[结果]内蒙古赤峰地区4个辣椒主产区辣椒混合病样的sRNA高通量测序，共检测到 6种病毒，常规RT-PCR共验证到其中 4种病毒;4个地区病毒种类存在明显差异，松山区城子乡优势种类为蚕豆萎蔫病毒 2(Broad bean wilt virus 2，BBWV2)和马铃薯Y病毒(Potato virus Y，PVY)，喀喇沁旗锦山镇为PVY和辣椒轻斑驳病毒(Pepper mild mottle virus，PMMoV);宁城县和喀喇沁旗西桥镇为BBWV2;复合侵染类型以 2种病毒复合侵染为主，复合侵染率最高达 61。54%。对测定的序列系统发育分析表明，赤峰地区 4个辣椒产区的PMMoV和BBWV2存在遗传分化，PVY可能出现了新的遗传型。[结论]内蒙古赤峰地区不同辣椒主产区发生的病毒种类、检出率和病毒复合侵染种类均存在明显差异。
Identification and Genetic Evolution of Viruses Infecting Chili Peppers at Chifeng,Inner Mongolia
[Objective]Viruses that infected chili pepper crops in Chifeng City,Inner Mongolia were identified,and their genetic relationship analyzed.[Methods]The 47 infected chili pepper plants at 4 major producing areas in Chifeng City were diagnosed,and viruses identified by sRNA high-throughput sequencing with bioinformatic analysis and RT-PCR.Fragments of RT-PCR were sequenced using the Sanger method to conduct a phylogenetic analysis with the MEGA software.[Results]Six viruses were identified by sRNA high-throughput sequencing.Of which,4 were re-verified by RT-PCR.The dominant viruses infecting the 4 regions differed significantly.They were Broad bean wilt virus 2(BBWV2)and Potato virus Y(PVY)in Chengzi County of Songshang District,PVY and Pepper mild mottle virus(PMMoV)in Jinshan County of Kalaqin District,and BBWV2 in Ningcheng County and Xiqiao County of kalaqin District.When the infection was caused by multiple viruses,it was mostly by two major pathogens and had the highest rate of occurrence at 61.54%.The phylogenetic analysis on the sequenced nucleotides of the viruses showed distinctive genetic differences between PMMoV and BBWV2 and,possibly,PVY of a novel genetic type.[Conclusion]There are significant differences in the types of viruses,detection rates,and types of virus co-infections that occur in different chili production areas of Chifeng City in Inner Mongolia.

Chifeng City of Inner Mongoliachili pepper viral diseasesRNA sequencingRT-PCRmultiple species infectionphylogenetic analysis

周艳芳、王秀芝、张晓梅、罗香文、刘泽红、崔聪聪、孟令强、孙军玲

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赤峰市农牧科学研究所,内蒙古 赤峰 024031

湖南省农业科学院,湖南 长沙 410125

湖南省慈利县高峰土家族乡农业综合服务中心,湖南 张家界 427205

内蒙古赤峰地区 辣椒病毒病 sRNA高通量测序 RT-PCR 复合侵染 系统发育分析

国家大宗蔬菜产业技术体系赤峰综合试验站湖南省自然科学基金国家自然科学基金湖南省科技创新项目湖南省科技创新项目

CARS-23-G212021JJ30415320723832022CX082023CX39

2024

10.19303/j.issn.1008-0384.2024.07.009

福建农业学报
福建省农业科学院

福建农业学报

CSTPCD北大核心
影响因子：0.656
ISSN：1008-0384
年,卷(期)：2024.39(7)