[Objective]Viruses that infected chili pepper crops in Chifeng City,Inner Mongolia were identified,and their genetic relationship analyzed.[Methods]The 47 infected chili pepper plants at 4 major producing areas in Chifeng City were diagnosed,and viruses identified by sRNA high-throughput sequencing with bioinformatic analysis and RT-PCR.Fragments of RT-PCR were sequenced using the Sanger method to conduct a phylogenetic analysis with the MEGA software.[Results]Six viruses were identified by sRNA high-throughput sequencing.Of which,4 were re-verified by RT-PCR.The dominant viruses infecting the 4 regions differed significantly.They were Broad bean wilt virus 2(BBWV2)and Potato virus Y(PVY)in Chengzi County of Songshang District,PVY and Pepper mild mottle virus(PMMoV)in Jinshan County of Kalaqin District,and BBWV2 in Ningcheng County and Xiqiao County of kalaqin District.When the infection was caused by multiple viruses,it was mostly by two major pathogens and had the highest rate of occurrence at 61.54%.The phylogenetic analysis on the sequenced nucleotides of the viruses showed distinctive genetic differences between PMMoV and BBWV2 and,possibly,PVY of a novel genetic type.[Conclusion]There are significant differences in the types of viruses,detection rates,and types of virus co-infections that occur in different chili production areas of Chifeng City in Inner Mongolia.
关键词
内蒙古赤峰地区/辣椒病毒病/sRNA高通量测序/RT-PCR/复合侵染/系统发育分析
Key words
Chifeng City of Inner Mongolia/chili pepper viral disease/sRNA sequencing/RT-PCR/multiple species infection/phylogenetic analysis
维普
万方数据