Impact of Different Modifiers on the Structure of Soil Bacterial Community in Red Soil Restoration Forest Land
The Pinus massoniana Lamb.restoration forest land in subtropical red soil area was chose for the investigation of the effects of different modifiers on the soil bacterial community structure to improve the soil quality and soil conservation effects in red soil restoration forest land.The undisturbed forest land(CK)and the application of organic modifiers(OF),biochar(BF)and composite modifiers(CF)were set up for the study of the soil chemical properties,the relative abundance and diversity of soil bacterial communities,so as to explore the responsiveness of soil fertility and bacterial community structure to different soil modifiers in erosion restoration forests in red soil areas,and to analyze the correlation between soil chemical properties and bacterial community structure.The results showed that:(1)different soil modifiers significantly increased soil pH and organic carbon content(P<0.05)by 4.20%~6.72%and 40.20%~102.37%,respectively.Of which,the organic modifiers increased soil total phosphorus content by 56.98%(P<0.05),biochar increased soil total nitrogen content by 73.09%(P<0.05),and composite amendments increased soil total phosphorus and total nitrogen content by 64.57%and 72.09%(P<0.05),respectively;(2)the dominant phyla of soil bacterial communities with different modifiers are Proteobacteria,Actinobacteria and Acidobacteria,while the dominant phyla is Acidobacteria,a-Proteobacteria and Actinobacteria;(3)all soil modifiers reduced the diversity of bacterial communities,while the application of biochar increased the richness of soil bacterial communities;(4)the total phosphorus content in soil has a significant impact on the composition of bacterial communities,and the organic carbon content is negatively correlated with the diversity of bacterial communities;and(5)the application of composite modifiers increased the relative abundance of metabolic pathways in soil bacterial communities.The results of this study can provide theoretical and data support for improving the soil fertility of forest land restoration in red soil areas,and for reasonably regulating the structure of bacterial communities.
soil and water lossred soilrestoration forest landsoil modifierbacterial community structurehigh-throughput sequencing