Effect of Pyrroloquinoline Quinone on Serum Metabolomics of Mice with Exercise-Induced Fatigue
Objective:To explore the effect of pyrroloquinoline quinone(PQQ)on serum metabolomics in mice with exercise-induced fatigue,seek out key markers,and uncover the metabolic pathway as well as the potential regu-latory mechanism of PQQ in alleviating exercise-induced fatigue.Methods:the mouse model of exercise-induced fatigue was established by repeated exhaustive swimming,and PQQ was supplemented for two weeks at a dose of 10 mg/kg per day.The metabolites in the serum of mice were detected by chromatography and mass spectrometry,and the key metabolites screened and their related metabolic pathways were analyzed in detail.Results:(1)after repeated exhaustion,there were 32 different metabolites in the serum of exercise-induced fatigue mice compared with normal quiet mice;(2)After repeated exhaustion,PQQ supplemented mice had 10 potential differential me-tabolites in serum compared with mice without PQQ supplementation;(3)Further comparison between groups re-vealed four significantly different metabolites in the two groups of mice,namely citric acid,xylitol,L-proline and n-acetyl-l-phenylalanine;(4)The main differential metabolic pathways included the glyoxylate cycle branch in the tricarboxylic acid cycle involving citric acid and the glyoxylate pathway involving xylitol.Conclusion:PQQ exerts a significant role in sustaining the normal serum metabolic level of mice with exercise-induced fatigue.,and its mechanism may be closely related to the regulation of key metabolites such as citric acid,xylitol,L-proline and n-acetyl-l-phenylalanine and their metabolic pathways.
万方数据
维普
