Rapid separation of ganoderic acid from the extracted by-products of Ganoderma lucidum
Objective To isolate the ganoderic acids from the extract-like product precipitated from the bottom of the concentration tank during the water extraction and production process of Ganoderma lucidum (G. lucidum). Methods The extract-like product precipitated at the bottom of the concentration tank in the production process of G. lucidum water extraction was dissolved in ethanol, sonicated, and centrifuged at high speed to obtain a supernatant. Three kinds of crude samples were obtained by using the preparation of Flash medium pressure, then prepared by Prep high pressure with detection tracking of HPLC. Results In turn, three samples of the same parts were collected, and white crystals were obtained by concentration and recrystallization, with the purity of over 98％, Three kinds of crystallization were identified respectively as Ganoderic acid C2, Ganoderic acid B and Ganoderic acid A by using GC-MS, IR, 1HNMR, and 13CNMR and other spectral means, combined with relevant literature datas. The extraction rates from by-products were ganoderic acid A: 1.62％-3.00％; ganoderic acid B: 1.83％ —2.59％; Ganoderic acid C2 : 1.03％-1.52％. Conclusion The use of rapid separation and purification of waste byproducts rich in ganoderic acid to prepare a reference substance provides a sufficient source for pharmacological research and detection of ganoderic acid.
Ganoderma lucidumprocessing by-products of Ganoderma lucidumholographic fast purification chromato-graphganoderic acidstructural identification
NETL
NSTL
万方数据
维普
