Clinical efficacy and mechanism of acupotomy in the treatment of knee osteoarthritis
Objective To observe the clinical effect of acupotomy on knee osteoarthritis and explore the mechanism of acupotomy on knee osteoarthritis.Methods Ten healthy people and forty patients with KOA were recruited and divided into normal group and acupotomy group.In the normal group,the joint effusion was extracted directly,and the acupotomy group was treated with acupotomy for 7 days,lasting for 3 courses.In the acupotomy group,10 patients were randomly selected to extract joint effusion before treatment and after 3 courses of treatment.Shear wave elastography(SWE),Visual analogue scale(VAS)and Western Ontario and McMaster Universities(WOMAC)were compared before and after acupotomy to evaluate the therapeutic effect.The expression of IL-1β and Caspase-1 protein in joint fluid were detected by Western blot,differential pro-tein screening and KEGG enrichment analysis.Results SWE value,VAS score and WOMAC score decreased significantly after treatment,and the differences were statistically significant(P＜0.05).The hardness of the rectus femoris muscle after treat-ment was lower than that before treatment.Acupotomy can down-regulate the expression of IL-1β and Caspase-1 protein in joint fluid of KOA patients,and play an anti-inflammatory and protective role in cartilage.KEGG enrichment analysis showed that the signal pathways involved in KOA lesions were mainly PPAR signal pathway and MAPK signal pathway.Conclusion Acu-potomy has good clinical efficacy in the treatment of knee osteoarthritis.At the same time,acupotomy can reduce the intraartic-ular inflammation by down-regulating the expression of protein targets in chondro-related signaling pathways.
high throughput proteomicsknee osteoarthritisacupotomy
万方数据
维普
