Neutrophil-derived exosomes promote migration and invasion of lung adenocarcino-ma cells
Objective To investigate the influence of neutrophil-derived exosomes on migration and invasion of lung ade-nocarcinoma cells.Methods All-trans retinoic acid(ATRA)was used to induce differentiation of human promyelocytic leuke-mia NB4 cells into neutrophils.The morphologic changes of neutrophils were analyzed by MGG staining,and the expressions of CD11b and CD18 for the neutrophils specific markers were detected by flow cytometry.The total exosome kit was used to isolate and extract exosomes derived from NB4 cells and neutrophils,the morphology and size of exosomes were identified by transmis-sion electron microscopy.The wound healing and Transwell assay were used to investigate the effects of NB4-derived and neu-trophil-derived exosomes on the migration and invasion of A549 cells.Results The NB4 cells were differentiated into neutrophils successfully and the expressions of CD11b and CD18 were significantly increased after ATRA induction.The NB4-derived and neutrophil-derived exosomes ranged from 40 nm to 100 nm,and were bilayer,hollow,round or oval.The NB4-de-rived and neutrophil-derived exosomes could promote the migration and invasion of A549 cells,and the effect of neutrophil-de-rived exosomes in promoting the migration and invasion of A549 cells was more pronounced.Conclusion Neutrophil-derived ex-osomes could promote the migration and invasion of lung adenocarcinoma cells significantly,and play a major role in the occur-rence and progression of lung adenocarcinoma.
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