Objective To explore the efficacy of nanocarriers based on mesoporous silica and polydopamine for delivering apatinib.Methods By utilizing the efficient drug delivery ability of mesoporous silica(MSN)and the pH responsiveness of polydopamine(PDA),mesoporous silica nanoparticles were prepared using an improved Stober method.A pH responsive polydopamine(PDA)coated mesoporous silica nanodrug delivery system was designed based on electrostatic composite method(MSNs@PDA).The success of MSNs@PDA system preparation was verified by scanning electron microscopy(SEM)and Zeta potential changes.The drug loading capacity and embedding rate of MSNs@PDA-Apa were determined.Vitro cytotoxicity was evaluated by the CCK-8 method.Transwell method was used to evaluate cell migration.Results MSNs@PDA system prepara-tion was successful,which was verified by scanning electron microscopy(SEM)and Zeta potential changes.The drug loading capacity of MSNs@PDA-Apa was 2.4％,and the embedding rate was 48％±2.5％.The cytotoxicity of MSNs@PDA-Apa group was concentration dependent,and when the concentration reached 10 μg/mL,the survival rate of PC-9 cells was only a-bout 30％.Compared to the Apa group,the effect of MSNs@PDA-Apa group on cell migration was significantly enhanced.Conclusion MSNs@PDA-Apa system preparation has a high drug delivery efficiency and exhibits a good killing effect on tumor cells,which is expected to provide ideas and methods for the construction of future drug delivery systems.
维普
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