Effect of GRWD1 on the proliferation and invasion of gastric cancer cells
Objective To investigate the effect of GRWD1(Glutamate-rich WD40 repeat containing 1)on the prolifera-tion and invasion ability of gastric cancer MKN-45 cells.Methods GRWD1 overexpression vector and siRNA were constructed and transfected into gastric cancer MKN-45 cells.GRWD1 overexpression group,siRNA group and control group were estab-lished.Cell proliferation levels in each group were measured using the CCK-8,and transwell was used to detect the effects of GRWD1 overexpression and gene silencing on the invasion ability of gastric cancer cell line MKN-45.Results The results showed that after transfection of MKN-45 cells with siRNA for 24 hours,the cell proliferation level and invasion ability were sig-nificantly inhibited(P＜0.01),while after transfection of MKN-45 cells with overexpression vector for 24 hours,the cell prolif-eration level and invasion ability were significantly enhanced(P＜0.01).Conclusion GRWD1 is related to the proliferation and invasion ability of MKN-45 gastric cancer cells,and downregulation of GRWD1 can inhibit the proliferation level and invasion a-bility of MKN-45 cells.On the contrary,upregulation of GRWD1 can improve the proliferation level and invasion ability of MKN-45 cells.
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