Correlation between expression of serum MicroRNA-16-5p and vascular endothelial growth factor and diabetic microangiopathy
Objective To investigate the correlation between serum levels of MicroRNA-16-5p(miR-16-5p)and vascular endothelial growth factor(VEGF)and diabetic microangiopathy(DMVC).Methods Type 2 diabetes(T2DM)patients admit-ted from June 2021 to June 2023 were selected as the study subjects.They were divided into the only diabetes(DM)group(n=20)and the diabetic microangiopathy(DMVC)group(n=51)based on whether they had microvascular complications,and healthy subject in the same period were selected as the negative control(NC)group(n=20).Clinical data of each group were analyzed and compared.The expression of serum miR-16-5p was detected by qRT-PCR and the expression of serum VEGF was detected by ELISA kit.Risk factors for developing microvascular complications in diabetic patients were analyzed.Results The DBP,FBG,LDL-C,and HbA1c levels in the DM and DMVC groups were higher than those in the NC group(P＜0.05).The BMI,SBP,and TG levels in the DMVC group were higher than those in the NC group(P＜0.05).The serum miR-16-5p level in the DMVC group was lower than that in the NC and DM groups,while the serum VEGF level was higher(P＜0.05).Pear-son correlation analysis showed that serum miR-16-5p expression was negatively correlated with VEGF(P＜0.05).Logistic re-gression analysis showed that VEGF and miR-16-5p were influencing factors for the occurrence of microvascular complications in diabetic patients.The ROC curve analysis showed that the area under the curve(AUC)for serum miR-16-5p,VEGF,and their combination in predicting the occurrence of microvascular complications in diabetic patients were 0.841,0.702,and 0.837,re-spectively.Conclusion Decreased expression of serum miR-16-5p is associated with the occurrence and development of diabetes and is a risk factor for microvascular complications.miR-16-5p and VEGF can serve as potential biomarkers for predicting the occurrence of microvascular complications in diabetic patients.
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