Effect of shRNA silencing circ_0089153 on proliferation,migration and invasion of TPC-1 thyroid cancer cells
Objective To investigate the effects of circ_0089153 on the proliferation,migration and invasion of TPC-1 thyroid cancer cells.Methods The expression of circ_0089153 in TPC-1 cells was silenced by circ_0089153 shRNA and divi-ded into blank control group,negative control group and shRNA silenced group.The expression of circ_0089153 mRNA and protein in TPC-1 cells in different groups was detected by RT-PCR and Western blot,the proliferation of TPC-1 cells in different groups was detected by CCK-8 method,and the migration and invasion ability of TPC-1 cells in different groups was detected by transwell assay.Results The mRNA and protein expression levels of circ_0089153 in shRNA silencing group were lower than those in blank control group and negative control group(P＜0.05).After silencing hsa_circ_0089153,TPC-1 cell proliferation was inhibited.The number of cell proliferation,migration and invasion in shRNA silencing group was lower than that in control group(P＜0.05).Conclusion circ_0089153 is closely related to the proliferation,migration and invasion of thyroid cancer cells.
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