Effects of NCAPG on the proliferation and apoptosis of colorectal cancer cell HCT116
Objective To investigate the effect and mechanism of Non-SMC condensin I complex subunit G(NCAPG)on the growth,proliferation and apoptosis of colorectal cancer cells(CRC).Methods siRNA-NCAPG was transfected into hu-man colorectal cancer cell line HCT116 cells.The effect of si-NCAPG on the expression of NCAPG in HCT116 cells was detec-ted by Western blot.The effect of si-NCAPG on the growth of HCT116 cells was evaluated by observing cell morphology and cell viability,and the best sequences were screened for subsequent experiments.The effects of si-NCAPG on the cell growth,cycle and apoptosis of HCT116 cells were evaluated by clonal formation,cell cycle,apoptosis,and western blot.Results si-NCAPG significantly decreased the expression of NCAPG protein level(P＜0.05),inhibited the cell number and cell viability(P＜0.05),induced cell cycle arrest in G2/M phase(P＜0.05)and promoted cell apoptosis in HCT116 cells(P＜0.05).Knocking down NCAPG reduced expression levels of Cyclin B1,CDK1 and Bcl-2 protein and increased expression levels of BAX protein.Conclusion si-NCAPG significantly inhibits the growth of CRC by inducing cell cycle arrest at G2/M phase and promo-ting cell apoptosis.
万方数据
维普
