Study on targeted delivery and apoptosis effects of self-assembled liposome nano-carriers on osteosarcoma cells Saos-2
Objective Using natural fatty acids lauric acid and stearic acid as the backbone,a liposome nanocarrier was constructed through their self-assembly in dimethylformamide(DMF)and loaded with doxorubicin.Its targeting ability to hu-man osteosarcoma cells Saos-2 and apoptosis were investigated.Methods Doxorubicin-loaded liposome nanocarriers were pre-pared by self-assembly.The targeting ability of the nanocarriers to human osteosarcoma cells Saos-2 was examined using confo-cal laser scanning microscopy.The effect of liposome nanocarriers on the apoptosis of human osteosarcoma cells Saos-2 was in-vestigated using Annexin V-FITC staining technique.Results When the self-assembled liposome nanocarriers were incubated with human osteosarcoma cells Saos-2 for 48 hours,the fluorescence intensity was the highest.The green fluorescent self-as-sembled liposome nanocarriers surrounded the osteosarcoma cells Saos-2.Compared to the control group with the same dose of DMF,both early and late apoptotic cell proportions increased in the self-assembled liposome nanocarrier group.Conclusion Doxorubicin-loaded liposome nanocarriers can accurately target human osteosarcoma cells Saos-2 and accelerate their apoptosis.
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