基于体外微量反应评价某些甾醇类化合物对5α还原酶的抑制作用
Evaluation of the inhibitory effects of certain sterol compounds on 5 α-reductase based on in vitro micro reactions
林滨 1蔡沓栗 1何小蓉 1何宇 1江举1
作者信息
- 1. 福州市皮肤病防治院,福州 350025
- 折叠
摘要
目的 基于体外微量反应,体外筛选有效的5α还原酶抑制物.方法 从SD大鼠肝脏中获得5α还原酶的粗酶,建立体外微量反应体系,通过反应前后睾酮量的变化来评价受试甾醇类化合物对5α还原酶的抑制作用.结果 在反应体系中加入二甲基亚砜(DMSO),解决非那雄胺及受试甾醇类化合物在水相中溶解度低等的问题,确保微量反应有效进行,从而得到可靠的筛选结果.结论 羊毛甾醇在体外具有较好的5α还原酶抑制效果,或许可以成为新型的5α还原酶抑制物.
Abstract
Objective To screen effective inhibitors of 5 α-reductase in vitro,based on in vitro micro reactions.Methods The crude enzyme of 5 α-reductase was obtained from the liver of SD rats,and then established a micro reaction system in vitro.The inhibitory effect of the tested sterol compounds on 5 α-reductase was evaluated by the changes in testosterone levels before and after the reaction.Results The addition of dimethyl sulfoxide(DMSO)to the reaction system solved the problem of low solubility of finasteride and tested sterol compounds in the aqueous phase,ensuring effective trace reactions and obtaining reliable screening results.Conclusion Lanosterol has a good inhibitory effect on 5 α-reductase in vitro,and may become a new type of 5 α-reductase inhibitor.
关键词
5α还原酶/非那雄胺/体外/脱发Key words
5 α-reductase/finasteride/in vitro/hair loss引用本文复制引用
基金项目
福州市科学技术局医疗卫生项目(2022-S-070)
福州市"十四五"临床专科培育建设项目(20220303)
出版年
2024
NETL
NSTL
万方数据