Construction of UCNPs@mSiO2-PEG nanomaterials and cellular targeting ability
Objective To prepare UCNPs@mSiO2-PEG nanomaterials and investigate their ability to target colon cancer CT26 cells.Methods Mesoporous silica was coated on upconversion materials to prepare coreshell structured nanoparticles(UC-NPs@mSiO2).Polyethylene glycol(PEG)modification was carried out on its surface.The constructed UCNPs@mSiO2-PEG nanomaterials were characterized by X-ray diffraction(XRD)and transmission electron microscopy(TEM).The ability to target colon cancer CT26 cells was observed by laser confocal photography.Results UCNPs@mSiO2-PEG showed an amorphous SiO2 characteristic diffraction peak at 2θ～22°.UCNPs@mSiO2-PEG nanoparticles were in a single dispersed spherical shape.The SiO2 film layer covered the surface of UCNPs nanoparticles,and the PEG modification layer further covered the SiO2 film layer.The UCNPs@mSiO2-PEG carrier emitting green fluorescence surrounded the colon cancer CT26 cells.Conclusion The coreshell structured nanoparticles UCNPs@mSiO2 were successfully constructed and surface-modified.The modified UCNPs@mSiO2-PEG nanoparticles can effectively target colon cancer CT26 cells.
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