Diagnostic value of peripheral blood metagenomic next-generation sequencing tech-nology in patients with malignant hematologic diseases complicated with fever
Objective To investigate the diagnostic value of peripheral blood metagenomic next-generation sequencing(mNGS)in patients with malignant hematologic diseases complicated with fever.Methods Twenty-three peripheral blood mNGS results from patients with malignant hematologic diseases complicated with fever who were hospitalized in Fujian Provin-cial Hospital from June 2021 to June 2023 were restrospectively analyzed,and compared with traditional blood culture tests.The characteristics of the pathogenic spectrum and the efficacy of anti-infection were analyzed.Results Among the 23 patients detec-ted by peripheral blood mNGS technology,9 cases were diagnosed of viral infection,4 cases of bacterial infection,2 cases of fun-gal infection,3 cases of mixed viral and bacterial infection,and 1 case of mixed viral and fungal infection.Only three cases de-tected in traditional peripheral blood culture were diagnosed of bacterial infection,mainly Klebsiella pneumoniae and Acineto-bacter baumannii,but no fungi and viruses were detected.The positive detection rate of mNGS in peripheral blood was signifi-cantly higher than that in traditional blood culture(82.6％vs.13.0％,P＜0.05).The effective rate of targeted anti-infective therapy based on the results of peripheral blood mNGS was significantly higher than that of traditional empirical anti-infective therapy(73.6％vs.25.0％,P＜0.05).Conclusion Compared with traditional blood culture,peripheral blood mNGS technol-ogy has higher positive detection rate than traditional blood culture in the etiological diagnosis of patients with malignant hemato-logic diseases complicated with fever,which is helpful to improve the clinical anti-infection efficacy.
万方数据
维普
