Mechanism exploration of reverse targeting of microRNA-24 by PTEN inhibitors to inhibit the occurrence and development of cervical cancer
Objective To investigate the role of microRNA-24(miR-24)in the pathogenesis,formation and regulation of cervical cancer(CC).Methods Subcutaneous tumor formation test of nude mice was conducted with HELA S3 cell line.The expressions of Cyclin D1,Ki-67,E-cadherin and Vimentin were detected by immunohistochemistry(IHC)to study the effects of miR-24 and PTEN on tumor growth.Results The tumor weight was significantly inhibited in miR-24 inhibitor group.The expression levels of Cyclin D1 and Ki-67 decreased,and cell proliferation was inhibited.E-cadherin expression was enhanced,while Vimentin expression was inhibited.The detection results of miR-24 inhibitor+si-PTEN group showed that si-PTEN re-versed the effect of miR-24 inhibitor.Conclusion miR-24 inhibitors significantly inhibit the proliferation and invasion of CC cell lines,and down-regulation of PTEN can reverse the effects of miR-24 inhibitors on the proliferation and invasion of CC cell lines.Targeting miR-24 may provide a new therapeutic strategy for the prevention and treatment of CC.
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