Effects of Xuling-Jiangu Decoction on Bone Metabolism and Local Angiogenesis in Ovariectomized Rats
Objective:To explore the effect of Xuling-Jiangu decoction on bone metabolism and local angiogenesis in ovariecto-mized rats through hypoxia inducible Factor-1α(HIF-1α)/vascular endothelial growth factor(VEGF)pathway.Methods:Twenty four 3-month-old rats were randomly divided into sham operation group,model group,Xuling group and estradiol group,with 6 rats in each group.Only part of the fat around both ovaries were removed in the sham operation group,and both ovaries were removed in the other three groups.One week after removal,the Xuling group was given 1 mL/(100 g·d)solution containing 1.5 g/mL crude drug by gavage,and the estradiol group was given 1 mL/(100 g·d)solution containing 0.0125 mg/mL estradiol by gavage,the sham operation group and the model group were given the same amount of saline by gavage,once a day.After 12 weeks of intervention,bone mineral density of the left tibia was detected,trabecular area percentage(Tb.Ar%)and trabecular width(Tb.Wi)and trabecular separation(Tb.Sp)of the left distal femur were measured.Serum levels of estradiol(E2),Procollagen I N-terminal Propeptide(P1NP)and type I col-lagen cross-linked C-terminal peptide(S-CTX)were detected.The expression levels of differentiation determinant antigen 31(CD31),HIF-1α and VEGF were detected by immunohistochemistry.Results:Compared with sham operation group,bone mineral density of the left proximal tibia in the model group decreased significantly(P<0.01),Tb.Wi of the left distal femur had no significant change(P>0.05),Tb.Ar%decreased,Tb.Sp increased significantly,E2 and P1NP levels in model group decreased,while S-CTX levels in-creased,the expression levels of HIF-1α and VEGF in the left distal femur significantly decreased(P<0.05).Compared with the mod-el group,bone mineral density of rats in the Xuling group and the estradiol group increased,Tb.Wi had no significant change(P>0.05),Tb.Ar%increased and Tb.Sp decreased,serum E2 and P1NP levels increased(P<0.05),while S-CTX decreased only in the Xul-ing group(P<0.05).The expression levels of CD31,HIF-1α and VEGF in left distal femur of the Xuling and estradiol groups signifi-cantly increased(P<0.05).There were no significant differences in the above indexes between the Xuling and estradiol groups(P>0.05).Conclusion:Xuling-Jiangu decoction can up-regulate HIF-1α/VEGF expression in distal femur bone tissue,promote angio-genesis and bone formation,stabilize bone metabolism,and reduce bone loss in ovariectomized rats.
万方数据
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