Effect of Liqi Tongbian Formula on Intestinal Motility in Slow Transit Constipation Rats with Qi Stagnation Syndrome Based on SCF/c-kit Signaling Pathway
Objective:To investigate the effect of Liqi Tongbian formula on intestinal motility in slow transit constipation(STC)rats with Qi stagnation syndrome based on SCF/c-kit signaling pathway.Methods:Thirty-six female Wistar rats were divided into control group(n=6)and modeling group(n=30)using the random number table method.In the modeling group,"loperamide suspen-sion + tail pinch stimulation"was used to replicate the STC rats with Qi stagnation syndrome model for 14 days.Successfully mod-eled rats were randomly divided into model group,western medicine group,low-dose group,medium-dose group,and high-dose group,with 6 rats in each group.the low-,medium-,and high-dose groups were administered with Liqi Tongbian formula solution by gavage at doses of 5.15,10.3,and 20.6 g/(kg·d),respectively;the western medicine group was administered with Prucalopride succi-nate suspension by gavage at doses of 0.18 mg/(kg·d);the control and model groups were given sterile water by gavage at doses of 10 mL/(kg·d),once a day,continuously gavage for 14 days.The quantity of fecal excretion,fecal water content,and small intestine propulsion rate were detected 6 hours after the last administration;the pathological changes of colon tissue was observed by HE staining;the tyrosine kinase growth factor receptor(c-kit)protein expression level of colon tissue was measured by immunohisto-chemistry;the c-kit and stem cell factor(SCF)protein expression of colon tissue was measured by Western blot.Results:HE stain-ing showed that the colonic mucosa of rats in the control group was intact,with well-arranged cup cells and glands,and no aggrega-tion of inflammatory cells was observed;in the model group,the colonic mucosa appeared to have poorly arranged intestinal glands,and the submucosal mesenchymal vasculature was dilated;in each of the administered groups,the intestinal glands of the colonic mucosa were well-arranged,and no obvious epithelial damage was observed.Compared with the control group,the quantity of fecal excretion,fecal water content,and small intestine propulsion rate in the model group significantly decreased(P<0.05),and the expres-sion level of c-kit protein and the expression of c-kit and SCF proteins in colonic tissues significantly decreased(P<0.05).Compared with the model group,the quantity of fecal excretion,fecal water content,and small intestine propulsion rate significantly increased in the western medicine,medium-dose,and high-dose groups(P<0.05);the quantity of fecal excretion,fecal water content signifi-cantly increased in the low-dose group(P<0.05);the expression level of c-kit protein in colonic tissues of the administered groups all significantly increased(P<0.05);the expression of c-kit protein in colonic tissues of the low-,medium-and high-dose groups all sig-nificantly increased(P<0.05),and the expression of SCF protein in the high-dose group significantly increased(P<0.05).Conclu-sion:Liqi Tongbian formula can increase the expression of ICC and regulate the SCF/c-kit signaling pathway in the colon tissue of STC rat models with Qi stagnation syndrome,thus restoring the normal regulation of gastrointestinal rhythm to improve the symp-toms of constipation.
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