Effect and Mechanism of Zibai Ointment in Promoting Wound Repair
Objective:To explore the effect and mechanism of Zibai ointment in promoting wound repair in rats.Methods:1)ani-mal experiment:a full-layer incision was made on the back of the rats and staphylococcus aureus was used to establish the wound red-ness and swelling model.The rats with successful modeling were randomly divided into blank group,vaseline group and Zibai oint-ment group,with 7 rats in each group.The vaseline group was externally coated with vaseline,and the Zibai ointment group was exter-nally coated with Zibai ointment,with a dosage of 3 g.The wound was cleaned and bandaged to prevent infection,and dressing was changed daily until the wound was completely healed.The color and texture of the wound were observed,and the area of the wound was measured.The mRNA expression level of vascular endothelial growth factor(VEGF)and angiopoietin-1(Ang-1)was detected by RT-qPCR.2)Cells experiment:HUVEC cells were divided into DMSO group,1 mg/mL group,5 mg/mL group,and 10 mg/mL group.The DMSO group was intervened with DMSO solution,while the 1,5,and 10 mg/mL groups were intervened with 1,5,and 10 mg/mL Zibai ointment paste suspension,respectively,for 48 hours.MTT assay was used to detect the cells viability after 12,24 and 48 hours of intervention;the cell migration ability was detected by scratch healing test;the transwell migration and invasion assay was used to detect the migration and invasion ability of cells;the mRNA expression level of VEGF and Ang-1 was detected by RT-qPCR.Results:1)animal experiment:Compared with the blank group and vaseline group,the wound area of Zibai ointment treatment group signifi-cantly decreased after 4 days of treatment(P<0.05).Compared with the blank group,the mRNA expression level of VEGF and Ang-1 in vaseline and Zibai ointment groups significantly increased(P<0.05).Compared with the vaseline group,the mRNA expression level of VEGF and Ang-1 in Zibai ointment group significantly increased(P<0.05).2)Cell experiment:Compared with the DMSO group,the cells viability of 1 mg/mL group significantly increased after intervention for 12,24 and 48 hours(P<0.05),and 5,10 mg/mL groups significantly increased after intervention for 12,24,36,and 48 h(P<0.05).Compared with the DMSO group,the cell migra-tion rate in 1,5 and 10 mg/mL groups significantly increased(P<0.05);the number of cells attacked and migrated significantly in-creased(P<0.05);the mRNA expression level of VEGF and Ang-1 mRNA significantly increased(P<0.05).Conclusion:Zibai oint-ment can accelerate wound healing by promoting wound angiogenesis.
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