首页|五苓散通过NF-κB/NLRP3信号通路抑制糖尿病肾病小鼠肾纤维化

五苓散通过NF-κB/NLRP3信号通路抑制糖尿病肾病小鼠肾纤维化

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目的 探究五苓散对糖尿病肾病db/db小鼠肾纤维化的改善作用及机制.方法 将24只8周龄db/db小鼠按照体质量随机分成模型组、五苓散组、厄贝沙坦组,每组8只,另取8只同周龄db/m小鼠为正常组.五苓散组给予五苓散7.2 g/(kg·d)灌胃,厄贝沙坦组给予厄贝沙坦22.5 mg/(kg·d),正常组和模型组db/db小鼠给予等体积生理盐水,连续给药8周.观察4组小鼠一般情况,检测糖化血清蛋白(GSP)、24 h尿蛋白定量(24 h-UTP)、甘油三酯(TG)、总胆固醇(TC)、血肌酐(SCr)及尿素氮(BUN)含量;Masson染色观察肾组织病理改变;Western blot、qPCR法检测肾组织中核因子NF-κB p65、核苷酸结合寡聚化结构域样受体蛋白3(NLRP3)mRNA表达水平和蛋白表达量.结果 与正常组比较,模型组db/db小鼠体质量及GSP、24 h-UTP、TG、TC、SCr、BUN含量均明显升高(P<0.05);肾小球体积增大,基底膜增厚,肾间质大量胶原纤维沉积;肾组织中NF-κB p65、NLRP3 mRNA表达水平及蛋白表达量均明显升高(P<0.05);与模型组比较,五苓散组db/db小鼠治疗后体质量及GSP、24 h-UTP、TG、TC、SCr、BUN含量均明显降低(P<0.05),厄贝沙坦组db/db小鼠24 h-UTP、SCr、BUN含量均明显降低(P<0.05);五苓散组和厄贝沙坦组db/db小鼠肾小球基底膜增厚明显改善,肾间质胶原纤维沉积明显减少,肾组织中NLRP3、NF-κB p65 mRNA表达水平及蛋白表达量均明显降低(P<0.05).结论 五苓散可能通过调控NF-κB/NLRP3信号通路,抑制糖尿病肾病db/db小鼠肾脏组织纤维化.
Wuling Powder Inhibits Renal Fibrosis in Diabetic Nephropathy Mice via NF-κB/NLRP3 Signaling Pathway
Objective:To investigate the effect and mechanism of Wuling Powder on renal fibrosis in db/db mice with diabetic ne-phropathy.Methods:According to body weight,24 8-week-old db/db mice were randomly divided into model group,Wuling Powder group and irbesartan group,8 db/db mice in each group,and 8 db/m mice of the same age were selected as normal group.The Wuling Powder group was given Wuling Powder 7.2 g/(kg·d)by gavage,the irbesartan group was given irbesartan 22.5 mg/(kg·d),db/db mice in the normal group and model group were given equal volume normal saline,all groups were intervened for 8 weeks.The general situation of mice were observed,the content of glycated serum protein(GSP),24 hour urine total protein quantity(24 h-UTP),tri-glyceride(TG),total cholesterol(TC),serum creatinine(SCr),and blood urea nitrogen(BUN)were tested;the pathological changes of renal tissue were observed by Masson staining;Western blot and qPCR were used to detect the mRNA and protein expression of nuclear factor NF-κB p65 and NOD-like receptor protein 3(NLRP3)in renal tissue.Results:compared with the normal group,the body weight,the content of GSP,24 h-UTP,TG,TC,SCr,and BUN of db/db mice in the model group increased significantly(P<0.05);the volume of glomerulus increased,the glomerular basement membrane thickened,and a large amount of collagen fibers de-posited in renal interstitium;the mRNA and protein expression of NF-κB p65 and NLRP3 in renal tissues increased significantly(P<0.05).Compared with the model group,the body weight,the content of GSP,24 h-UTP,TG,TC,SCr,and BUN of db/db mice in the Wuling Power group decreased significantly(P<0.05)after treatment,the content of 24 h-UTP,SCr and BUN of db/db mice in the irbesartan group significantly decreased(P<0.05);the glomerular basement membrane thickening of db/db mice in the Wuling Powder and irbesartan groups was significantly improved,and the collagen fibers deposition in renal interstitium reduced significantly,the mRNA and protein expression of NF-κB p65 and NLRP3 in renal tissues significantly decreased(P<0.05).Conclusion:Wuling Pow-der may inhibit renal fibrosis in db/db mice with diabetic nephropathy by regulating NF-κB/NLRP3 signaling pathway.

diabetic nephropathyrenal fibrosisWuling PowderNF-κB/NLRP3 signaling pathway

陈彦君、林阳榕、詹绘苗、陈勇

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福建中医药大学中西医结合学院,福建 福州 350122

糖尿病肾病 肾纤维化 五苓散 NF-κB/NLRP3信号通路

国家自然科学基金项目福建省自然科学基金项目福建省大学生创新创业训练计划项目福建省大学生创新创业训练计划项目

819738272021J01890S202210393019202210393012

2024

福建中医药
福建省中医药学会 福建中医药大学

福建中医药

影响因子:0.518
ISSN:1000-338X
年,卷(期):2024.55(6)
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