Mechanism of Pien Tze Huang Inhibiting Epithelial-Mesenchymal Transition on Human Pancreatic Cancer Cells
Objective:To explore the regulatory effect of PZH on the proliferation,migration and epithelial-mesenchymal transi-tion (EMT) of AsPC-1 and PANC-1 cells. Methods:The MTT assay was used to screen the optimal concentrations of PZH solution for intervention in AsPC-1 and PANC-1 cells as 0,0.5,and 1 mg/mL. Transcriptome sequencing was used to detect gene expression changes in AsPC-1 cells intervened with 0,1 mg/mL PZH;wound healing experiment was used to detect the migration ability of AsPC-1 and PANC-1 cells;transwell migration assay was used to detect the migration ability of AsPC-1 and PANC-1 cells;Western blot was used to detect the protein expression of E-cadherin,Vimentin,N-cadherin,and Zinc finger E-box binding homeobox 1 (ZEB1) in AsPC-1 and PANC-1 cells. Results:1) Transcriptome sequencing:There were significant differences in gene expression levels between the 0 and 1 mg/mL groups in important indicators related to EMT. 2) Scratch healing experiment:Compared with the 0 mg/mL group,the scratch damage rate of PANC-1 cells in the 1 mg/mL group significantly reduced after 12 hours (P<0.05),the scratch damage rate of AsPC-1 cells in the 1 mg/mL group significantly reduced after 24 hours (P<0.05),and the scratch damage rates of AsPC-1 and PANC-1 cells in the 0.5 and 1 mg/mL groups significantly reduced after 48 hours (P<0.05). 3) Transwell experi-ment:Compared with the 0 mg/mL group,the relative migration ratio of AsPC-1 and PANC-1 cells in the 1 mg/mL group significantly reduced (P<0.05). 4) Western blot experiment:Compared with the 0 mg/mL group,the protein expression of E-cadherin of AsPC-1 cells in the 1 mg/mL group significantly increased (P<0.05),while the protein expression of Vimentin of AsPC-1 cells in the 0.5 and 1 mg/mL groups significantly reduced (P<0.05);compared with the 0 mg/mL group,the protein expression of N-cadherin and ZEB1 of PANC-1 cells in the 1 mg/mL group significantly reduced (P<0.05). Conclusion:PZH obviously inhibits the proliferation,migra-tion and EMT of human pancreatic cancer cells,which has the potential effect of anti-metastasis in pancreatic cancer.