Objective:To evaluate the safety of Taxus chinensis fruit(TCF)extract by acute toxicity test in mice.Methods:A to-tal of twenty female and twenty male C57BL/6 mice were randomly divided into normal group and TCF group based on gender.The TCF group was given TCF extract solution by gavage at a dose of 6 g/(kg·d),while the normal group was given physiological saline solution by gavage at a dose of 10 mL/(kg·d)for 14 consecutive days.The growth,development and mortality of mice were ob-served and recorded,and weighed once a week.The organs were dissected and weighed,and the indexes of liver,spleen,kidney and brain were calculated.Serum alanine transaminase(ALT),aspartate transaminase(AST),total protein(TP),albumin(ALB),blood urea nitrogen(BUN),creatinine(CREA),glucose(GLU),high density lipoprotein(HDL),low density lipoprotein(LDL),triglyceride(TG)and total cholesterol(TC)were detected;HE staining was used to observe the pathological changes in liver,heart,kidney,large intestine,and small intestine.Results:During the period of gavage,the mice in each group grew and developed well without obvious abnormal behavior,poisoning symptoms,and death.There was no significant difference in body weight of different genders between the two groups before intervention and on the 7th and 14th day of intervention(P>0.05).There were no significant differences in the indexes of liver,spleen,kidney and brain between the two groups of different genders(P>0.05).HE staining showed that the liver,kid-ney,heart,large intestine,and small intestine tissues of the two groups were intact and structurally clear,and no obvious pathological changes were found.Conclusion:At a dose of 6 g/(kg·d),no significant toxic side effects were observed with TCF,indicating its good safety.
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